糖尿病肾病患者AR基因表达量测定  

Determination of AR Gene Expression in Diabetic Nephropathy

作　　者：何永鑫[1] 李雪[2] 范雪梅[3] 王琬[3] 王义明[1] 罗国安[1,3] 

机构地区：[1]清华大学化学系,北京100084 [2]清华大学医学院,北京100084 [3]华东理工大学药学院,上海200237

出　　处：《高等学校化学学报》2010年第2期293-295,共3页Chemical Journal of Chinese Universities

基　　金：国家"九七三"计划项目(批准号:2005CB523503)资助

摘　　要：对50例正常人和80名糖尿病肾病不同分期的患者进行醛糖还原酶(Aldose Reductase,AR)基因的表达量测定,提示AR基因有望成为糖尿病肾病(DN)早期诊断的生物标志物以及DN治疗上潜在的药物靶点,并且利用AR基因对DN的中医诊断进行了分子生物学验证.Diabetic nephropathy（DN） is a common chronic diabetic microvascular complication.DN is closely related to polyol pathway of glycometabolism,and aldose reductase（AR） is a rate-limiting enzyme in polyol pathway.In order to study the AR expression level in patients with different stages of DN,AR gene expression was detected in 50 cases of normal subjects and 80 diabetic nephropathy patients with different stages.The results show that AR gene is expected to be a biomarker for early diagnosis of DN,as well as the potential drug target in DN treatment.According to AR gene expression,the diagnosis of diabetic nephropathy in Chinese medicine is verified on the molecular biology level.

关 键 词：糖尿病肾病 醛糖还原酶 Taqman实时荧光定量PCR 

分 类 号：O657[理学—分析化学] Q344.13[理学—化学]