胆囊组织、组织细胞膜及细胞株的FTIR研究  

Studies on Gallbladder Tissues,Tissue Plasma Membranes and Cell Lines by FTIR

作　　者：王健生[1] 张佳[1] 吴文安[1] 段小艺[1] 王嗣岑[2] 张明鑫[1] 周苏娜[1] 莫非[1] 徐怡庄[3] 石景森[1] 吴瑾光[3] 

机构地区：[1]西安交通大学第一医院肿瘤外科,西安710061 [2]西安交通大学天然药物研究中心,西安710061 [3]北京大学化学与分子工程学院,北京100871

出　　处：《高等学校化学学报》2010年第3期484-487,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:30500119);国家“九七三”计划前期专项基金(批准号:2002CCA01900)资助

摘　　要：研究了不同病理生理状态的胆囊组织、胆囊组织细胞膜及胆囊癌细胞株的傅里叶变换红外光谱的相关性.结果显示,与正常组织比较,胆囊癌组织中脂质相关谱带(2955,2920,2870,2850和1740 cm-1)与蛋白相关谱带(1550 cm-1)强度降低;胆囊癌细胞株中脂质相关谱带与蛋白相关谱带(1550 cm-1)强度明显增加;胆囊癌组织细胞膜中脂质相关谱带强度明显增加;核酸相关吸收谱带强度变化在胆囊癌组织和胆囊癌细胞株中一致,在细胞膜中消失;I1460/I1400比值在组织、细胞株和细胞膜层面变化一致,与正常组织相比均有所减少.结果表明,与胆囊癌组织相比,胆囊癌细胞株和胆囊癌组织细胞膜的红外光谱发生了显著变化:癌组织的脂类和蛋白质的相对含量降低;癌细胞株的脂质和蛋白相对含量增加;胆囊癌组织细胞膜的脂质和蛋白相对含量升高.从组织、细胞和细胞膜3个层面阐述了癌变的分子光谱变化.I1460/I1400比值的变化在组织、细胞株和细胞膜层面变化一致,对此指标的深入研究有望获得一个良好的临床诊断指标.12 Cancer tissue samples,14 inflammatory samples and 10 normal samples of gallbladder and their corresponding tissue membranes and gallbladder cancer cell lines were observed via FTIR.Compared to normal gallbladder tissue,the intensities of bands,related to lipids,at 2955,2920,2870,2850 and 1740 cm-1,decreased for gallbladder cancer tissue,the intensity of bands,related to protein,at 1550 cm-1 decreased for gallbladder cancer tissue;the intensities of lipids bands and protein bands increased for gallbladder cancer cell line obviously;the intensities of lipids related bands also increased for gallbladder cancer tissue membrane;the intensity of band related to nucleic acid for gallbladder cancer tissue is similar to that for gallbladder cancer cell line and the band disappeared for gallbladder cancer tissue plasma membrane;the ratio of I1460/I1400 changed uniformity for tissue,cell line and tissue plasma membrane,which is smaller for cancer ones and bigger for normal ones.These results indicate that compared with those of gallbladder cancer tissue, the FTIR spectra changed a lot for gallbladder cancer cell lines and gallbladder cancer tissue plasma membrane.Protein and lipids relatively decreased in gallbladder cancer tissues but increased in gallbladder cancer cell line and gallbladder cancer tissue plasma membrane.The paper expounds FTIR variation for carcinogenesis from tissue,cell line and tissue plasma membrane.The ratio of I1460/I1400 changed uniformity for tissue,cell line and tissue plasma membrane,more studies may get a fine clinical diagnosis index.

关 键 词：胆囊 胆囊癌 细胞膜 胆囊癌细胞株 红外光谱 

分 类 号：O657.3[理学—分析化学]