鸡MyoG基因在成纤维细胞中的表达  

Expression in chick embryo fibroblast cell of MyoG gene

作　　者：张婷婷[1] 胡兰[1] 王宏艳[1] 袁亚琦[1] 郝文博[1] 杨晓宇[1] 

机构地区：[1]沈阳农业大学畜牧兽医学院,沈阳110161

出　　处：《东北农业大学学报》2010年第3期93-96,共4页Journal of Northeast Agricultural University

基　　金：辽宁省教育厅科研基金资助项目(2007304)

摘　　要：利用分子克隆技术,通过RT-PCR反映扩增、克隆、测序含有Eco RⅠ/XhoⅠ酶切位点的MyoG片段,构建真核荧光表达载体pEGFP-N1-MyoG,并通过脂质体瞬时转染鸡成纤维细胞,对表达产物进行RT-PCR和SDS-PAGE鉴定。结果表明,经脂质体介导重组体pEGFP-N1-MyoG可成功转染鸡成纤维细胞,RT-PCR和SDS-PAGE电泳证实pEGFP-N1-MyoG可在鸡成纤维细胞中表达出MyoG蛋白。该结果为进一步研究MyoG基因的生物学作用奠定了基础。MyoG cDNA containing Eco R I/Xho I sites was amplified by RT-PCR, and then cloned and sequenced. Eukaryotic expression vector pEGFP-N1-MyoG was transfected into chick embryo fibroblast cells with lipidosome, and the expression protein was detected by SDS-PAGE. The result showed that MyoG gene was cloned into pEGFP-N1 successfully, and its expression at mRNA and protein level was detected with RT-PCIR and SDS-PAGE. The experiment established the basis for further study on the function of MyoG gene.

关 键 词：MYOG 基因克隆 真核表达 

分 类 号：S813[农业科学—畜牧学] Q78[农业科学—畜牧兽医]