猪SRPK1基因的电子克隆  

Silicon cloning of pig SRPK1 gene

作　　者：俄广鑫[1] 刘娣 谢雨彤[1] 祝继原[1] 杨少成[1] 王嘉博[1] 赵金凤[1] 

机构地区：[1]东北农业大学动物科学技术学院,哈尔滨150030 [2]黑龙江省农业科学院畜牧研究所,哈尔滨150086

出　　处：《东北农业大学学报》2010年第3期101-104,共4页Journal of Northeast Agricultural University

基　　金："十一五"国家科技支撑计划重点项目(2008BADB2B01)

摘　　要：用小鼠和人的SRPK1基因的编码区序列通过NCBI数据库进行比较和检索,得到一个高同源性的猪cDNA序列和4个猪SRPK1基因的ESTs。利用DNAMAN软件将以上片段进行拼接,得到了一条较长的拼接片段X-1。ORF finder和Blast 2 sequence程序分析。结果表明,X-1中包含一个完整的长为1 968 bp的编码区,编码656个氨基酸,且此编码区DNA序列与人的SRPK1基因有91.73%的一致性,氨基酸序列的一致性为92.93%。Use the SRPK1 sequence of human and mouse to compare and search in NCBI date, where a high similar cDNA sequence and four pigs＇ SRPK1 ESTs were found, which were spliced into a longer one （X-1） by DNAMAN software, A complete opening frame of 1 968 bp length, was found by ORF finder and Blast 2 sequence in the X-1 sequence, which encoded a protein of 656 amino acids. This coding sequence of 1pin-old showed 91.73% indentity in 1 968 bp segments with the human SRPK1 gene and had 92.93% identity in 656 amino acids.

关 键 词：SRPK1 生物信息学 电子克隆 开放读码框查找 

分 类 号：S828[农业科学—畜牧学]