海南粗榧外植体灭菌与愈伤组织诱导  

Surface Sterilization and Callus Induction of Stem Explants of Cephalotaxus hainanensis Li

作　　者：刘进平[1,2] 吴佳静[2] 黄艳丽[2] 周志刚[2] 张维[2] 魏丹萍[2] 张科立[2] 

机构地区：[1]海南大学热带作物种质资源保护与开发利用教育部重点实验室 [2]海南大学农学院,海南儋州571737

出　　处：《热带农业科技》2010年第1期39-41,共3页Tropical Agricultural Science & Technology

摘　　要：对海南粗榧茎段外植体灭菌和愈伤组织诱导实验研究表明,以海南粗榧温室扦插苗的嫩茎作为外植体,70%酒精1min,0.1%氯化汞溶液12min表面消毒处理效果最好;最适愈伤组织诱导培养基为MS+0.5mg/LNAA+0.5～1.0mg/L BA+2.5～4.0mg/L 2,4～D。Cephalotaxus mannii Hook.f.（Cephalotaxus hainanensis Li） is a recalcitrant plant for in vitro culture due to its severe contamination, browning and slow growth. The young shoots of Cephalotaxus hainanensis Li cultivated in glasshouse were used as explants and the explants were sterilized in 70% ethanol for 1 min following with dipping in 0.1%HgCl2 solution for 12 min and rinsed three times in sterilized water. In vitro response of explants was tested with different combinations of BA （6-benzylamino purine）, NAA （naphthalene acetic acid） and 2,4-D （2,4-dichlorophenoxy acetic acid）. The optimum effect of callus induction was obtained in the medium of MS ＋ 0.5 mg/L NAA ＋ 0.5-1.0 mg/L BA ＋ 2.5-4.0 mg/L 2,4-D.

关 键 词：海南粗榧 组织培养 愈伤组织 灭菌 

分 类 号：S791.53[农业科学—林木遗传育种]