利用TAC系统构建多个光合同化物运输蛋白基因转化载体和水稻的遗传转化  

Application of multigene expression vector containing transformation-competent artificial chromosome and agrobacterium-mediated transformation for studying their effects on rice

作　　者：苏军[1] 林美坤[1] 杜琳[1] 洪海强[1] 王锋[1] 

机构地区：[1]福建省农业科学院农业遗传工程重点实验室,福建福州350003

出　　处：《福建农业学报》2010年第1期1-7,共7页Fujian Journal of Agricultural Sciences

基　　金：福建省自然科学基金项目(2006J0004)

摘　　要：采用人工染色体(TAC)多基因组装载体系统,将一组与水稻光合同化物运输转化有关基因构建在同一载体上,这些基因分别是蔗糖转运蛋白基因(OsSUT5),单糖转运蛋白(OsMST4)、细胞壁转化酶(OsCIN3)和ATP/ADP转运蛋白(OAAT),获得了包含这4个基因的植物表达载体pTAC747H-OAAT-SUT5-CIN3-MST4,质粒大小近30 kb。通过农杆菌转化法,将基因导入水稻,以探讨这些基因对水稻光合同化物运输和转化的调控作用。By using a transformation- competent artificial chromosome vector pYLTAC747H, we constructed four genes relating to assimilation translocation, including OsSUTS, OsMST4, OsCIN3 and OAAT in the expression vector, pTAC747H - OAAT - SUT5 - CIN3 - MST4. The entire inserted fragment was approximately 30 kb. By the agrobacterium-mediated transformation, 7 independent clones were obtained. PCR detection showed that 4 target genes and one marker gene had been integrated into the rice genome.

关 键 词：水稻 多基因表达载体 遗传转化 

分 类 号：Q78[生物学—分子生物学] S511[农业科学—作物学]