规模化猪场仔猪腹泻4种病原的感染情况调查  

Four diarrhea pathogens in piglets on large farms

作　　者：陈强[1] 曾丽莉[1] 俞伏松[1] 叶鼎承[1] 杨金先[1] 李艳虹[1] 宋铁英[1] 

机构地区：[1]福建省农业科学院生物技术研究所,福建福州350003

出　　处：《福建农业学报》2010年第1期8-13,共6页Fujian Journal of Agricultural Sciences

基　　金：福建省发改委项目(2008-762);科技部科技人员服务企业行动项目(2009GJC40042)

摘　　要：采用PCR和RT-PCR技术,对福建省16个规模化猪场的仔猪腹泻样本进行致病性大肠杆菌(EPEC)、猪传染性胃肠炎病毒(TGEV)、猪流行性腹泻病毒(PEDV)和猪轮状病毒(RDV)的感染情况调查。结果表明:15个猪场EPEC检测为阴性,1个猪场同时检测到大肠杆菌K88的201 bp条带和仔猪水肿病大肠杆菌F18ab菌毛蛋白的510 bp条带,EPEC的阳性率为6.25%。1个猪场检测到TGEV的497 bp特有条带,TGEV的阳性率为6.25%。3个猪场检测到PEDV的854 bp特有条带,PEDV的阳性率为18.75%。3个猪场检测到RDV的342 bp特有条带,RDV的阳性率为18.75%。1个猪场同时感染EPEC和PEDV 2种病原,其余的15个猪场均为单一病原感染。表明福建省规模化猪场的仔猪腹泻仍然存在EPEC、TGEV、PEDV、RDV病原感染,呈现散发流行,与以往的报道相比阳性率明显下降。PCR and RT - PCR were used to investigate the infection caused by enteropathogenic Escherichia coli （EPEC）, transmissible gastroenteritis virus of swine （TGEV）, porcine epidemic diarrhea virus of swine （PEDV） and rotavirus of swine （RDV） in piglets from 16 large - scale swine farms in Fujian in 2009. The result showed that only one farm was infected with the 201bp band of E. coli K88 and the 510bp band of F18ab fimbriae of E. coli eliciting edema disease of piglet. EPEC was not found in other farms. The positive rate of EPEC was 6.25%. There was a farm, in which the 497bp band of TGEV was found with a positive rate of 6.25%. Three farms were found with the 854bp band of PEDV or 342bp band of RDV. Hence, the positive rates of TGEV and RDV were both 18.75%. It was concluded that the EPEC, TGEV, PEDV and RDV diarrhea infection continued to exist in piglets in the large-scale swine farms in Fujian. However, the infection showed up only in separate areas, and the rates were significantly reduced as compared to the previous reports.

关 键 词：仔猪腹泻 致病性大肠杆菌 猪传染性胃肠炎病毒 猪流行性腹泻病毒 猪轮状病毒 PCR RT—PCR 

分 类 号：S858.28[农业科学—临床兽医学] S852.612[农业科学—兽医学]