柴胡多糖的分子量测定及单糖组成分析  

Molecular Weight Determination and Monosaccharide Composition Analysis of Bupleurum chinense DC Polysaccharide

作　　者：颜军[1] 刘嵬[1] 邬晓勇[1] 郭晓强[2] 何钢[2] 张良蕾[2] 孙晓春[2] 苟小军[1] 

机构地区：[1]成都大学中药化学实验室,四川成都610106 [2]成都大学药用微生物重点实验室,四川成都610106

出　　处：《安徽农业科学》2010年第9期4550-4552,共3页Journal of Anhui Agricultural Sciences

基　　金：四川省中医药管理局科技专项资助项目(2008-01);四川省教育厅科研项目资助(08ZA173)

摘　　要：[目的]从柴胡中提取分离柴胡精多糖(BPP2),并对其分子量和单糖组成进行研究。[方法]采用水提法提取柴胡精多糖(BP),用DEAE纤维素柱色谱进行分离纯化,采用GPC法测定BPP2的分子量,TLC、HPLC法对其单糖组成进行研究。[结果]BPP2的分子量约为67836,由鼠李糖(Rha)、木糖(Xyl)、阿拉伯糖(Ara)、半乳糖(Gal)和葡萄糖(Glc)5种单糖组成,其摩尔比为2.19∶1.00∶3.21∶2.27∶2.44。[结论]柴胡多糖BPP2是由5种单糖组成的纯一杂多糖,分子量为67836。[Objective] The aim was to extract and isolate Bupleurum chinense DC polysaccharide （BPP2）,and study its molecular weight and monosaccharide composition. [Method] BPP2 was extracted from B. chinense with water,isolated and purified by DEAE ion-exchange cellulose. The molecular weight of BPP2 was measured using GPC method,and TLC,HPLC were adopted to study its monosaccharide composition. [Result] The molecular weight of BPP2 was 67 836,which was composed of Rha,Xyl,Ara,Gal and Glc with molar ratio of 2.19∶1.00∶3.21∶2.27∶2.44. [Conclusion] BPP2 is pure heterosaccharide which is composed of five monosaccharides,the molecular weight is 67 836.

关 键 词：柴胡多糖 分子量 单糖 组成 

分 类 号：R284.1[医药卫生—中药学]