Dot-ELISA间接法检测鸡新城疫病毒(NDV)的研究  

Studies on the Indirect Dot ELISA for the Detection of Newcastle Disease Virus

作　　者：曹洪敬[1] 王文志[1] 崔锦鹏[1] 任素芳[1] 颜世敢[1] 徐学孟 沈冰[1] 秦良勇[1] 

机构地区：[1]山东省农科院畜牧兽医研究所

出　　处：《中国动物检疫》1998年第6期1-3,共3页China Animal Health Inspection

摘　　要：常规方法分离纯化鸡ＩｇＧ，免疫羊制备羊抗鸡ＩｇＧ二抗，以辣根过氧化物酶（ＨＲＰ）标记制备酶标记物的工作浓度为１：１０００；选用硝酸纤维素（ＮＣ）膜作固相载体，建立检测ＮＤＶ抗原的斑点间接酶联免疫吸附试验诊断方法。检测ＮＤＶ鸡胚毒４０份，阳性检出率１００％；人工感染非免疫鸡的气管组织、肺组织各４７份，检出率为９３．６％；人工感染ＳＰＦ鸡气管组织、肺组织各４０份，检出率１００％。与传染性支气管炎病毒（ＩＢＶ）、减蛋综合症病毒（ＥＤＳＶ）、传染性法氏囊病病毒（ＩＢＤＶ）无交叉反应。试验显示该方法简便、特异、快速、结果直观。Goat anti. chicken IgG was prepared by immunization of goat with purified chicken IgG,and conjugated with horse radish peroxidase (HRP).The working concentration of the HRP conjugated IgG was 1∶1000. An indirect dot ELISA was developed using nitrocellulose (NC)filter as solid carrier. 40 chicken embryoes with NDV were detected resulting in 100% positive detection rate. 47 trachea and lung samples from experimentally infected non vaccinated chickens gave 93.6% positive rate and 40 trachea and lung samples from experimeutally infected SPF chickens gave 100% detection rate. No cross reactions were seen when it was used to detect IBV ,EDSV and IBDV.It was proved that the indirect dot ELISA for NDV was simple, specific and rapid and the result could be read directly.

关 键 词：鸡 新城疫病毒 DOT-ELISA 

分 类 号：S858.315.3[农业科学—临床兽医学]