猪鸡致病性大肠杆菌沙门菌对β-内酰胺类药耐药表型和基因检测  

Detection of β-lactamases-resistant genes in Salmonella and Escherichia coli isolated from swines and chickens

作　　者：叶满玉[1] 王红宁[1] 田国宝[1] 张安云[1] 赵应望[1] 周英顺[1] 

机构地区：[1]四川大学生命科学学院动物疫病防控与食品安全四川省重点实验室,四川成都610064

出　　处：《中国兽医杂志》2010年第3期15-18,共4页Chinese Journal of Veterinary Medicine

基　　金：国家"十一五"科技支撑计划重大项目(2006BAK02A19);国家农业科技成果转化基金(05EFN215100243)

摘　　要：本试验指130株细菌对β-内酰胺类药物头孢噻吩、头孢西丁、头孢他啶、头孢曲松、头孢噻肟等的耐药表型进行研究。结果大肠杆菌及沙门菌对β-内酰胺类耐药率较高:头孢噻吩83.5%、头孢他啶63.4%。敏感率较高的:头孢曲松93.2%、亚胺培南97%、头孢吡肟100%。采用本实验室建立的三重PCR方法,检测β-内酰胺类药物CTX-M、SHV和TEM耐药基因,TEM的检出率最高82%(82/130),SHV的检出率34%(34/130)、CTX-M检出率23%(23/130)。耐药基因和耐药性比较表明,大肠杆菌及沙门菌对β-内酰胺类药物耐药表型与耐药基因型符合率较高,能很好的反映细菌耐药情况并给临床用药提供参考。Antibiotic sensitivity test was carried out in 130 strains.β-lactamases-resistant genes TEM,SHV,and CTX-M were detected by the multiplex-PCR.The results showed that the cephalothin resistance rate was 83.5%,the ceftazidime resistance rate was 63.4%.The imipenem sensitive rate was 100%,the cefepime sensitive rate was 97% and the ceftriaxone sensitive rate was 93.2%.The result of the antibiotic resistance gene showed that the TEM antibiotic resistance gene rate is 84.6%,the SHV antibiotic resistance gene rate is 41.5% and the CTX-M antibiotic resistance gene rate is 34.6%.The result may be of help to clinical treatment.

关 键 词：Β-内酰胺类药物 大肠杆菌 沙门菌 耐药基因 检测 

分 类 号：S859.796[农业科学—临床兽医学]