狂犬病病毒HepFlury株P蛋白单克隆抗体的制备及间接免疫荧光检测方法的建立  

Development of monoclonal antibodies against P protein of rabies virus

作　　者：毛三英[1] 郭霄峰[1] 何敏[2] 马勇江[1] 范小龙[1] 剡海阔[1] 马红娜[1] 邓衔柏[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]广东科贸职业学院,广东广州510430

出　　处：《中国兽医杂志》2010年第3期18-21,共4页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金项目(30671565)

摘　　要：本研究以原核表达的融合型的重组蛋白pET32a（＋）-P（狂犬病病毒HepFlury株P蛋白）免疫6-8周龄的BALB/c小鼠,取脾细胞和SP2/0按常规杂交瘤技术进行细胞融合,经间接免疫荧光方法（IFA）筛选及对mAb进行初步鉴定。经3次亚克隆后获得1株稳定分泌表达的杂交瘤细胞株5G8。The fusion protein（pET32a（＋）-P）was purified by TrapFF crude,6-8-week-old BALB/c mice were immunized with the protein mixed with adjuvants before the splenic cells of immunized mice were fused with SP2/0 myeloma cells.Indirect fluorescence antibody detection assay was used to screen hybridoma cells for the specific monoclonal antibodies.One hybridoma（5G8） producing monoclonal antibody were obtained after three rounds of subcloning.

关 键 词：重组蛋白pET32a(+)-P 细胞融合 间接免疫荧光方法(IFA) MAB 

分 类 号：S852.659.5[农业科学—基础兽医学]