高效液相色谱内标法检测鸡血浆中甲砜霉素的浓度  

DETERMINATION OF THIAMPHENICOL CONCENTRATION IN CHICKEN PLASMA BY HPLC WITH INTERNAL STANDARD

作　　者：张小华[1,2] 陶冠红[2] 陈园[1] 陈晓兰[1] 王妲妲[1] 

机构地区：[1]江苏畜牧兽医职业技术学院药物检测中心,泰州225300 [2]苏州大学化学化工学院,苏州215123

出　　处：《化学分析计量》2010年第2期48-51,共4页Chemical Analysis And Meterage

摘　　要：以氟甲砜霉素作内标，乙腈作为提取溶剂，采用高效液相色谱内标法检测鸡血浆中甲砜霉素的浓度。色谱柱为Shim—pack CLC—ODS（150mm×6mm，5um），流动相为乙腈-水（体积比25：75），流速为1．0mL／min，检测波长为225nm，柱温为40℃。在此色谱条件下，在0．25～32．00mg／L浓度范围内，甲砜霉素浓度与甲砜霉素和氟甲砜霉素峰面积比呈线性关系，相关系数r为0．9999，最低检测浓度为0．1mg／L；在高、中、低3个浓度水平下日内、日间精密度均大于6．1％（n=5），提取回收率大于98．68％，方法回收率为99．20％～100．25％。建立的方法符合生物样品的分析要求。可用于临床药代动力学研究。The blood concentrations in chicken of thiamphenicol were determined by the HPLC internal standard method, after the plasma containing florfenicol as the internal standard was extracted by acetonitrile. The chromatographic column was Shim -pack CLC - ODS column （ 150 mm × 6 mm, 5 um）, the mobile phase was acetonitrile - water （ 25 ： 75 ） at a flow rate of 1.0 mL/min, the detecting wavelength was 225 nm, and the column temperature was 40℃. In the chromatographic conditions, the calibration curve of thiamphenicol had a good linearity within a concentration range of 0.25 - 32.00 mg/L （ r = 0.999 9） and the detection limit was 0.1ug/mL. The intra - day and inter - day RSD （n = 5） were less than 6.1% at low, medium and high concentration levels. The extraction recoveries were more than 98.68% and the method recoveries were 99.20% - 100.25%. The method can meet the analysis requirements of biological samples, and it can be used for the study of clinical thiamphenicol pharmacokinetic.

关 键 词：甲砜霉素 高效液相色谱 氟甲砜霉素 内标 血浆 

分 类 号：O657.72[理学—分析化学]