玉米蛋白激酶基因ZmCIPK的序列及表达特性分析  

Analysis of sequence and expression characteristic of the CBL-interacting protein kinase from maize(ZmCIPK)

作　　者：李会云[1] 王伟[1] 胡秀丽[1] 王琦[1] 邰付菊[1] 

机构地区：[1]河南农业大学生命科学学院,河南郑州450002

出　　处：《河南农业大学学报》2010年第1期12-18,27,共8页Journal of Henan Agricultural University

基　　金：河南农业大学引进人才科研启动基金项目(30500247)

摘　　要：以玉米为材料,采用序列分析及半定量RT-PCR等方法对ZmCIPK基因功能进行了初步研究.序列分析结果推测,AY104819,EU974290和EU968441均属于CIPK蛋白家族.半定量RT-PCR结果表明,NaCl和ABA均能迅速诱导玉米基因AY104819,EU974290和EU968441的表达.Sequence analysis and semi-quantitative RT-PCR were used to study the preliminary function of the CBL-interacting protein kinase from maize（ZmCIPK）.From the result of sequence analysis,it is speculated that,AY104819,EU974290 and EU968441 all belong to CIPK protein family.Semi-quantitative RT-PCR analysis indicated that,maize gene AY104819,EU974290 and EU968441 can be rapidly induced by NaCl and ABA.

关 键 词：玉米幼苗 ZmCIPK 表达特性 序列分析 半定量RT-PCR 蛋白激酶基因 表达量 信号转导途径 分析结果 氨基酸序列 

分 类 号：S513[农业科学—作物学] Q786[生物学—分子生物学]