毛泡桐悬浮细胞培养及其植株再生  

Cell suspension cultures of Paulownia tomentosa and its plantlet regeneration

作　　者：翟晓巧[1,2] 胡文远[1] 范国强[1] 

机构地区：[1]河南农业大学,河南郑州450002 [2]河南省林业科学研究院,河南郑州450008

出　　处：《河南农业大学学报》2010年第1期34-40,46,共8页Journal of Henan Agricultural University

基　　金：国家自然科学基金项目(30571496);中国博士后科学基金项目(20070410874)

摘　　要：先将毛泡桐叶片在MS,WPM,KM8P和B5等液体基本培养基中进行悬浮培养,然后建立毛泡桐叶片悬浮细胞培养及其体外植株再生体系.结果表明,毛泡桐叶片愈伤组织悬浮诱导最适培养基为改良MS+0.2 mg.L-1NAA+8 mg.L-1BA;细胞悬浮培养的最佳起始密度为6.67×106个.mL-1;悬浮愈伤组织芽诱导和根诱导的最适培养基分别为MS+0.3 mg.L-1NAA+17 mg.L-1BA和1/2MS+0.1 mg.L-1NAA.The suspension cell line and system of its in vitro plantlet regeneration from the leaves of Paulownia tomentosa were established through optimization of the mineral and organic substances in MS media supplemented with various NAA and BA concentrations on the basis of callus induction rates in the basal liquid MS,WPM,KM8P and B5.The results indicated that the optimal medium of callus induction from the leaves of Paulownia tomentosa were modified liquid MS＋0.2 mg·L-1NAA＋8 mg·L-1 BA;and the best primary density for the cell culture was 6.67×106;The optimal ones of shoot and root induction from the induced calli in the modified liquid MS media were MS＋0.3 mg·L-1NAA＋17 mg·L-1BA and 1/2MS＋0.1 mg·L-1NAA,respectively.

关 键 词：毛泡桐叶 悬浮细胞培养 培养基 体外植株再生 愈伤组织诱导率 质量浓度 芽诱导 植物激素 细胞悬浮培养 外植体 

分 类 号：S792.43[农业科学—林木遗传育种]