急性特发性血小板减少性紫癜患儿外周血血小板相关抗体和白细胞介素18的检测  

Detection and clinical significance of interleukin-18 and platelet associated IgG in peripheral blood of acute idiopathic thrombocytopenic purpura children

作　　者：金呈强[1] 董海新[1] 刘仿[2] 宋丽[2] 赵元明[1] 孙传祥[1] 高岩[1] 高东田[1] 盛蕊[1] 

机构地区：[1]济宁医学院附属医院医学检验中心,山东济宁272029 [2]广东医学院微生物学与免疫学教研室,广东湛江524023

出　　处：《新乡医学院学报》2010年第1期48-51,共4页Journal of Xinxiang Medical University

基　　金：广东省自然科学基金资助课题(编号:06028959);东莞市科技计划项目资助课题(编号:2008108101033)

摘　　要：目的检测急性特发性血小板减少性紫癜(ITP)患儿治疗前后外周血血小板相关抗体(PAIgG)和白细胞介素18(IL-18)的变化,分析IL-18和PAIgG在急性ITP患儿发病机制中的作用。方法用流式细胞仪检测外周血PAIgG表达含量,ELISA法检测血浆中IL-18的含量,血细胞分析仪计数血小板(PLT)数量。结果急性ITP组治疗前PLT数量明显低于治疗后及对照组,差别均有统计学意义(P<0.01),治疗后PLT数量与对照组比较,差别无统计学意义(P>0.05);治疗前PAIgG表达量明显高于治疗后及对照组,差别均有统计学意义(P<0.01),治疗后的PAIgG表达量高于对照组,差别有统计学意义(P<0.05);治疗前IL-18含量均低于治疗后及对照组,差别有统计学意义(P<0.01),治疗后与对照组比较,差别无统计学意义(P>0.05)。PAIgG在治疗前后与血小板含量均呈负相关(r值分别为-0.87,-0.81,P<0.05),而IL-18含量在治疗前后与血小板含量均呈正相关(r值分别为0.76,0.74,P<0.05),IL-18含量和PAIgG表达在治疗前后均呈负相关(r值分别为-0.81,-0.79,P<0.05)。结论急性ITP患儿外周血中IL-18含量在治疗前后和PAIgG表达量有较好的相关性,IL-18的检测能够反映出急性ITP患儿外周血中PAIgG表达量的变化,PAIgG及IL-18在急性ITP发病机制中发挥了重要作用。Objective To investigate the changes of the concentration of interleukin-18（IL-18） and the variation of platelet associated IgG（PAIgG） in acute idiopathic thrombocytopenic purpura（ITP） children＇s peripheral blood,and analyze their roles in the pathogenesy of acute ITP furtherly.Methods The expressions of PAIgG were detected by the flow cytometry, and the contents of IL-18 were detected by enzyme linked immunosorbent assay,and the number of platelet（PLT） were detected by blood cell analyzer.Results Before treatment,the number of PLT in ITP group was significantly lower than after treatment and control group（P0.01）.After treatment,there was no statistic difference of PLT counts between ITP group and control group（P0.05）.Before treatment,the expressions of PAIgG in ITP group was significantly higher than after treatment and control group（P0.01）.After treatment,the expressions of PAIgG in ITP group were significantly higher than that in control group（P0.05）.Before treatment,the contents of IL-18 in ITP group was significantly lower than after treatment and control group（P0.01）.After treatment,there was no statistic difference of IL-18 levels between ITP group and control group（P0.05）.There were negative correlations between PAIgG and PLT counts before and after the treatment（r=-0.87,-0.81,P0.05）.There were positive correlations between IL-18 and PLT counts before and after the treatment（r =0.76,0.74,P0.05）.There were negative correlations between IL-18 and PAIgG before and after the treatment（r=-0.81,-0.79,P0.05）.Conclusions IL-18 contents have a better correlation with PAIgG in peripheral blood of acute ITP children.The detection of IL-18 can be a indicator to reflect the the expression of PAIgG in peripheral blood,PAIgG and IL-18 play an important role in the pathogenesis of acute ITP.

关 键 词：急性特发性血小板减少性紫癜 酶联免疫吸附测定 白细胞介素18 血小板抗体 

分 类 号：R725.5[医药卫生—儿科]