广东登革3型病毒株的分离培养和鉴定  

Culture, Isolation, and Identification of the Dengue Type 3 Virus Strain in Guangdong

作　　者：周伟泽[1] 张复春[1] 胡凤玉[1] 王建[1] 洪文昕[1] 

机构地区：[1]广州医学院附属市八医院,广州510060

出　　处：《热带医学杂志》2010年第2期141-144,共4页Journal of Tropical Medicine

基　　金：广州市科技支撑计划项目(No.2008Z1-E401)

摘　　要：目的对2009年发生于广东地区的3例家庭聚集性登革热患者血清进行登革病毒的鉴定和病毒株的培养分离。方法用胶体金法检测患者血清中登革病毒特异性IgM、IgG抗体;C6/36细胞培养患者血清中登革病毒;RT-PCR扩增C-PrM基因序列的片段以检测患者血清中登革病毒RNA,PCR产物经序列测定后进行生物信息学分析。结果3例患者血清学检测均为登革病毒特异性IgM阳性、IgG阴性;特异性RT-PCR产物长约290bp,经琼脂糖电泳和测序分析,证实3例患者血清中均存在登革3型病毒;从1例患者血清中培养分离到了登革病毒株,经RT-PCR和测序证实为登革3型病毒。结论2009年发生于广东地区的3例登革热患者经病毒培养和分子生物学鉴定,证实均为登革3型病毒感染。Objective To culture,isolate and identify the viral strain from serum of three clustering of dengue fever patients in a family in Guangdong in 2009. Methods Dengue virus specific IgM and IgG antibody were detected by Colloidal gold method. Dengue virus were cultured and isolated in C6/36 cell line. C-PrM gene was amplified by reverse transcriptase-polymerase chain reaction （RT-PCR）and the DNA sequence was determined for bioinformatics analysis.Results Dengue virus specific IgM antibody was positive while IgG antibody was negative in all three dengue patients＇ test.Length of specific product of RT-PCR was approximate 290 bp,and dengue virus type 3 were identified by agarose electrophoresis and sequencing.Dengue virus strain type 3 was cultured and isolated from one patient which was also identified by RT-PCR and sequencing. Conclusion The three dengue fever eases of Guangdong in 2009 were confirmed to be infected by dengue type 3 virus.

关 键 词：3型登革病毒 病毒分离 RT—PCR DNA序列分析 

分 类 号：R373.33[医药卫生—病原生物学]