高效液相色谱-电化学检测法测定人血浆中阿奇霉素的浓度  

Determination of azithromycin concentration in human plasma by HPLC-ECD

作　　者：潘熠斌[1] 陈醒言[1] 胡国新[1] 

机构地区：[1]温州医学院药学院药理学教研室,温州325035

出　　处：《中国临床药学杂志》2010年第2期96-98,共3页Chinese Journal of Clinical Pharmacy

摘　　要：目的建立测定人血浆中阿奇霉素浓度的高效液相色谱电化学（HPLC。ECD）检测法。方法采用ZORBAX XDB-C18柱（Agilent150mrn×4．6nm，5μm）进行分离；以乙腈-50mmol·L^-1 KH2PO4-K2HPO4缓冲液（pH=7．0）（70：30，WV）为流动相；流速为1．0mL·min^-1；柱温为45．0℃；ESA CoulochemⅢ型电化学检测器检测，电压650mV。以克拉霉素为内标，待测血浆经乙腈沉淀后，上清液氮气吹干，用定量流动相溶解后进样。结果本法线性关系良好（，=0．9999），线性范围10—1000μg·L^-1，定量下限为10μg·L^-1，回归方程p=6．1328Ai／As-0．0195；相对回收率均在85％-115％内，绝对回收率〉75％；日间、日内精密度RSD均〈10％。结论该法简便、灵敏、特异，适用于人体药动学及治疗药物检测的研究。AIM To develop a HPLC-ECD method for the determination of azithromyein concentration in human plasma. METHODS A ZORBAX XDB-C18 column （Agilent 150 mm × 4.6 mm, 5 μm）was used with a mobile phase consisting of phosphate buffer （ pH 7.0, 50 mmol·L^-1） -acetonitrile （70：30, V/V）. The flow rate was 1.0 mL·min^-1. The column temperature was 45.0℃ and the electric potential of ECD was 650 mV. Clarithromycin was used as the intemal standard. RESULTS The linear range of the plasma concentration of azithromycin was 10 - 100 0 μg·L^-1 and the limit of quantification was 10μg·L^-2. The regression equation was to = 6.132 8 Ai/As - 0.019 5, r = 0.999 9. The relative range of recovery was 85% - 115% and the absolute recovery of component added to plasma samples was more than 75%. Both the inter-day RSD and the intra-day RSD were less than 10%. CONCLUSION The method has high sensitivity and good selectivity, and it is suitable for therapeatic drug monitoring and the pharmacokinetic research of azithromycin in human plasma.

关 键 词：阿奇霉素 高效液相色谱-电化学检测法 人血浆 

分 类 号：R96[医药卫生—药理学]