密蒙花提取物滴眼剂对实验性干眼症大鼠泪腺组织细胞凋亡的影响(英文)  

Effects of eye drops of Buddleja officinalis Maxim. extract on lacrimal gland cell apoptosis in castrated rats with dry eye

作　　者：彭清华[1] 姚小磊[1] 吴权龙[1] 谭涵宇[1] 张菁蓉[1] 

机构地区：[1]湖南中医药大学第一附属医院中医眼科学重点学科,湖南长沙410007

出　　处：《中西医结合学报》2010年第3期244-249,共6页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金面上资助项目(No.30772824);教育部高等学校博士学科点专项基金资助项目(No.200805410004);湖南省自然科学基金资助项目(No.07JJ3049);湖南省科技厅科研基金资助项目(No.2009FJ3001);2008湖南省研究生创新基金资助项目(No.湘财教字[2008]68号);湖南省中医五官科学重点学科建设项目;国家中医药管理局中医眼科学重点学科建设项目

摘　　要：目的:通过检测密蒙花提取物滴眼剂对去势所致干眼症大鼠泪腺凋亡相关蛋白Bax和Bcl-2表达的影响,探讨密蒙花提取物滴眼剂治疗干眼症的作用机制。方法:将45只Wistar雄性大鼠随机分为假手术组、模型组和密蒙花提取物滴眼剂治疗组(简称治疗组)。模型组和治疗组大鼠行双侧去势术建立大鼠干眼症模型。治疗组大鼠用密蒙花提取物滴眼剂滴眼治疗,每次1滴,每天3次,模型组和假手术组大鼠用生理盐水滴眼。分别于治疗1、2和3个月后每组处死5只,取材用于相关指标检测。采用免疫组织化学法检测泪腺组织中凋亡相关蛋白Bax和Bcl-2的表达,并计数凋亡细胞数量。结果:治疗组治疗1、2和3个月后,泪腺导管及腺泡上皮细胞中Bax表达阳性的细胞数均明显低于模型组,Bcl-2表达阳性的细胞数均明显高于模型组,细胞凋亡数量均明显低于模型组(P<0.01)。结论:密蒙花提取物滴眼剂中主要成分为黄酮类。密蒙花提取物滴眼剂可显著抑制雄激素水平降低后大鼠干眼症的发生,抑制泪腺细胞凋亡。Objective： To explore the possible mechanism of eye drops of Buddleja officinalis extract in treating dry eye of castrated rats by analyzing the expressions of Bax and Bcl-2 proteins. Methods： Forty-five Wistar male rats were randomly divided into sham-operated group, untreated group and eye drops of Buddleja officinalis Maxim. extract （treatment） group. The dry eye model was established with orchiectomy in the untreated group and treatment group. Rats in the treatment group were treated with eye drops of Buddleja officinalis Maxim. extract, one drop once, three times daily. Eyes of rats in the shamoperated group and untreated group were instilled with normal saline. After one-, two-, or three-month treatment, five rats in each group were scarified respectively. Then samples were taken to detect related indices. Expressions of Bax and Bcl-2 of lacrimal gland were checked by immunohistochemical method and quantity of apoptotic cells was counted.Results： After one-, two- or three-month treatment, the quantities of expressions of Bax in acinar epithelial cells and glandular tube cells were significantly lower, and those of Bcl-2 were significantly higher in the treatment group than in the untreated group, and the quantities of apoptotic cells of the treatment group were significantly lower than those of the untreated group （P〈0.01）. Conclusion： The main components of extract of Buddleja officinalis Maxim. are flavonoids, which can significantly inhibit cell apoptosis in lacrimal gland.

关 键 词：睾丸切除术 干眼病 泪器 细胞凋亡 密蒙花 滴眼剂 大鼠 

分 类 号：R285.5[医药卫生—中药学]