用CHO—hTSHR细胞检测甲状腺刺激性抗体的研究  

Clinical value of thyroid-stimulating antibody measurement using CHO-hTSHR cell line

作　　者：周亚芹[1] 张洪梅[1] 董艳[1] 苏青[1] 

机构地区：[1]上海交通大学医学院附属新华医院内分泌代谢科,上海200092

出　　处：《中国医师杂志》2010年第2期169-171,共3页Journal of Chinese Physician

摘　　要：目的研究本课题组所构建的表达人重组TSH受体的中国仓鼠卵巢细胞（CHO—hT—SHR）在检测甲状腺刺激性抗体（TSAb）方面的临床应用价值。方法采用正常对照组及Graves病（GD）甲亢组血清的粗提IgG溶液来刺激CHO—hTSHR细胞，测定反应介质中的cAMP产量，计算TSAb活性。以正常对照组TSAb活性的孟＋2s作为正常上限值，根据该阳性判断标准来计算GD甲亢组TSAb的阳性检出率。结果GD甲亢组cAMP的产量及TSAb活性高于正常对照组[（353．65±126．34）pmol／LVS（237．21±77．15）pmol／L，（149．08±53．26）％ vs（100±32．52）％]，差异有统计学意义（P〈0．05）。采用该细胞测定的TSAb活性的正常上限值为165％，GD甲亢组TSAb的阳性检出率为50％（14／28）。结论本课题组所构建的CHO—hTSHR细胞株在检测GD甲亢患者血清中TSAb水平方面具有一定的临床应用价值。Objective To evaluate the clinical value of CHO-hTSHR cells in detecting thyroid stimulating antibody （TSAb）. Methods The cAMP production and TSAb activity were measured and cal- culated by stimulating CHO-hTSHR cell line with IgGs of normal control group and Graves＂ disease （GD） group. TSAb positive standard was set to more than the mean ＋ 2SD of TSAb activities in control subjects. The positive percentage of TSAb activity in GD group was calculated. Results The cAMP production and TSAb activities of GD group were higher than those of normal group [ （ 353.65 ± 126. 34 ） pmol/L vs （237.21 ± 77.15 ） pmol/L, （ 149. 08 ± 53.26） % vs （ 100 ± 32. 52） % , P 〈 0. 05 ]. The value that higher than 165% was set to be positive for TSAb. The positive percentage of TSAb in GD group was 50% （ 14/ 28 ）. Conclusion CHO-hTSHR cell line constructed by our group is suitable for detecting TSAb activity in the sera of patients with GD.

关 键 词：免疫球蛋白类 甲状腺刺激/分析 卵巢/细胞学 仓鼠 

分 类 号：R446.6[医药卫生—诊断学]