液氮冷冻法制作兔股骨头坏死模型:可行,理想与可信  

Establishment of rabbit femoral head necrosis models using liquid nitrogen refrigeration method:Is it feasible,ideal and confident?

作　　者：戴双武 丁帅[2] 李章华[2] 吴茂葵 奚江明 孟繁胜 熊文华 

机构地区：[1]深圳市宝安区石岩医院骨科,广东省深圳市518108 [2]武汉大学人民医院骨科,湖北省武汉市430060

出　　处：《中国组织工程研究与临床康复》2010年第2期276-279,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:目前缺乏一种理想股骨头坏死动物模型,从而制约了对其进一步的研究。目的:验证以液氮冷冻法制作兔股骨头坏死修复模型的可行行,为后续研究奠定基础。方法:取成年新西兰大白兔20只,双侧进行实验,在不切断股骨头圆韧带、髋关节不脱位的基础上,以液氮棉签连续快速冷冻股骨头25次,约10s/次。分别于术后第3,7天,2,4,6,8周拍双侧髋关节正位片X射线片、MRI,并进行股骨头标本的大体解剖学观察和组织学观察。结果与结论:在造模3d时的股骨头组织切片上,冷冻区和周围部的软骨细胞、骨细胞和骨髓组织呈现坏死。2周,可见坏死的骨小梁周围开始出现修复表现。4周,部分出现剥脱,6周,软骨剥脱更加明显,8周,呈骨性关节炎改变,关节软骨剥脱严重,有2例股骨头正常外形改变。证实非髋关节脱位情况下液氮冷冻股骨头,可以成功建立的兔股骨头坏死模型,简便易行成功率高,可作为其后续研究的理想模型。BACKGROUND：The deficiency of perfect animal femoral head necrosis model limited its further investigation. OBJECTIVE：To verify the feasibility of establishing rabbit femoral head necrosis models using liquid nitrogen refrigeration method,and to provide a foundation for subsequent research. METHODS：A total of 20 adult,New Zealand,white rabbits were selected in the study. The round ligament of femur was not cut off and femoral head was not dislocated,and the exposed femoral head were quick frozen using cotton bud carrying liquid nitrogen for successive 25 times,with 10 s per time. The specimens were examined by gross anatomy,X-ray film,MRI and histological observation at day 3,7 and weeks 2,4,6,and 8 after operation. RESULTS AND CONCLUSION：The histological section showed that chondrocyte,osteocyte,and myeloid tissues presented necrosis in freezing and periphery at 3 days after model preparation,and the repair process appeared at 2 weeks after operation. The articular surface of femoral heads appeared collapse at 4 weeks after operation,and these changes became obvious at 6 weeks. The femoral head presented osteoarthritis-like disorder,with seriously collapsed articular surface at 8 weeks,and the contour of femoral head changed in 2 animals. The results demonstrated that without hip dislocation,rabbit femoral head necrosis models can be established successfully using liquid nitrogen refrigeration method. This method is simple,feasible,with high succeed rate,which can be used in subsequent research.

关 键 词：冷冻 股骨头坏死 动物模型 实验造模 组织构建 骨组织工程 

分 类 号：R318[医药卫生—生物医学工程]