硬膜外移植自体髓核大鼠背根神经节的非压迫性损伤  

Autologous nucleus pulposus induces rat dorsal root ganglion damage without mechanical compression

作　　者：陆志东[1] 陈志荣[1] 

机构地区：[1]宁夏医科大学附属医院骨3科,宁夏回族自治区银川市750004

出　　处：《中国组织工程研究与临床康复》2010年第2期294-297,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:最新的研究表明,髓核所致的炎性反应是导致坐骨神经痛的重要原因之一,在此过程中背根神经节可能起重要作用,但是其病理生理变化目前尚不完全清楚。目的:探讨在无机械压迫情况下,腰椎间盘髓核突出引起坐骨神经痛的发病机制。方法:10月龄雄性SD大鼠24只,随机分成实验组和对照组,每组12只。切开大鼠尾椎椎间盘,髓核呈胶冻样,取5个髓核并加入50μL生理盐水,充分搅拌稀释成混悬液备用。实验组大鼠行硬膜外穿刺,注射混悬液到腰椎硬膜外腔制作动物模型。对照组硬膜外腔注射生理盐水。测定大鼠后肢机械刺激缩爪阈值并对疼痛相关行为和背根神经节组织形态学进行观察。结果与结论:在无机械压迫情况下,硬膜外移植自体髓核能使大鼠后肢产生明显的痛觉过敏,背根神经节会出现节细胞肿胀、核膜不清晰、核仁变淡或消失、胞浆出现空泡、尼氏小体颜色变浅分布不均匀、内膜间质增宽、充血和水肿等形态学改变。结果表明,移植自体髓核所致的炎性反应是引起大鼠背根神经节损伤和坐骨神经痛的重要原因之一。BACKGROUND：Resent studies demonstrate that sciatica is caused by inflammatory reaction of nucleus pulposus,and dorsal root ganglion （DRG） is in involved in this process; however,its pathophysiological changes remain poorly understood. OBJECTIVE：To explore pathogenesis of sciatica related to nucleus pulposus protrusion without mechanical compression. METHODS：Totally 24 male SD rats were randomly divided into the experimental group and control group,with 12 rats in each group. The coccygeal intervertebral discs of rats were opened to obtain 5 coccygeal nucleus pulposus,mixed with 50 μL physiological saline to prepare suspension. Rats in the experimental group were prepared non-compressive models by injecting suspension to the epidural cavum of rats. Meantime,the same volumes of physiological saline were injected into rats in the control group. The rat hind paw mechanical withdrawal thresholds were measured,pain-related behavior and dorsal root ganglion morphology were observed. RESULTS AND CONCLUSION：In the absence of mechanical pressure,disappearance of the nucleolus,degeneration of the nuclear membrane and significant hyperalgesia derived by auto-transplantation of nucleus pulposus to the epidural cavum of rats were observed. Dorsal root ganglion cells often had hyperemia,edema and intracytoplasmic vacuoles,with large and irregular nuclei. Prominent Nissl bodies were not seen. The Inflammatory reaction derived by autograft nucleus pulposus is an important factor in DRG injury and sciatica.

关 键 词：自体髓核 硬膜外注射 背根神经节 组织学 

分 类 号：R318[医药卫生—生物医学工程]