聚乙二醇-聚乙烯亚胺/四氧化三铁纳米磁流体的毒性及其生物相容性  

Toxicity and biocompatibility of polyethylene glycol-polyethyleneimine/ferroso-ferric oxide nano-magnetic fluid

作　　者：邹芬 潘一峰[2] 张红[2] 李国政[2] 唐静波[2] 彭健[2] 

机构地区：[1]中山市华南现代中医药城发展有限公司,广东省中山市528451 [2]中南大学卫生部纳米生物技术重点实验室,湖南省长沙市410008

出　　处：《中国组织工程研究与临床康复》2010年第3期447-451,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金项目(30672047);课题名称:治疗肝癌的自杀基因靶向磁纳米载体研究;湖南省研究生创新基金项目(2340-74335000013);课题名称:磁性纳米粒磁感应靶向性肿瘤热化疗仪的研究~~

摘　　要：背景:为实现纳米基因药物在治疗肿瘤中实现高靶向性,课题采用聚乙二醇和聚乙烯亚胺修饰的磁性四氧化三铁纳米粒(PEG-PEI/Fe3O4)作为基因药物载体,在提高载体载药能力的同时,使载体具有超顺磁性,增加药物靶向性能。目的:评价PEG-PEI/Fe3O4纳米磁流体体外和体内毒性。方法:对制备的PEG-PEI/Fe3O4纳米磁流体进行表征达到纳米材料水平后,过滤稀释5～20倍培养7702细胞和HpG2细胞,进行体外MTT毒性试验;通过体内溶血试验和微核试验测定生物相容性和体内毒性。结果与结论:MTT结果显示,PEG-PEI/Fe3O4纳米磁流体对7702细胞毒性0～1级,对正常肝细胞无害;PEG-PEI/Fe3O4纳米磁流体对HpG2细胞有轻微的旁观者抑制效应;PEG-PEI/Fe3O4纳米磁流体溶血率为0.372%,远小于5%;微核试验结果表明PEG-PEI/Fe3O4纳米磁流体无致畸、致突变作用。BACKGROUND：Polyethylene glycol-polyethyleneimine/ferroso-ferric oxide（PEG-PEI/Fe3O4） was selected as drug carriers in tumor treatment,which can increase drug loading capacity and targeting capacity.OBJECTIVE：To test the toxicity of PEG-PEI/Fe3O4 nano-magnetic fluid in vitro and in vivo.METHODS：When the prepared PEG-PEI/Fe3O4 nano-magnetic fluid reached nano level,7702 and HpG2 cell lines were filtrated and diluted in 5-20 multiple,and detected by in vitro MTT toxicity test assay;in vivo hemolysis test and micronucleus test was used to test the toxicity and biocompatibility.RESULTS AND CONCLUSION：MTT assay results indicated that the toxicity grade of PEG-PEI/Fe3O4 nano-magnetic fluid to 7702 cell line was 0-1,which was harmless to natural hepatic cells;however,PEG-PEI/Fe3O4 nano-magnetic fluid had slight bystander restraining effect to HpG2 cell line.Maximum hemolysis rate of the material was 0.372%,which was far less than 5%.The micronucleus test result indicated that PEG-PEI/Fe3O4 nano-magnetic fluid had no teratogenicity or mutagenicity.

关 键 词：磁流体 细胞毒性试验 生物相容性 溶血试验 微核试验 

分 类 号：R318[医药卫生—生物医学工程]