汉坦病毒Z5株M和S片段全基因序列测定及分析  

Full-length nucleotide sequence analysis of the S and M segments in Z5 strain of Hantavirus Z5 strain

作　　者：李婵[1] 谢荣辉[1] 朱函坪[1] 徐芳[1] 姚苹苹[1] 程胤凯[1] 朱智勇[1] 

机构地区：[1]浙江省疾病预防控制中心出血热重点实验室,杭州310051

出　　处：《中国人兽共患病学报》2010年第3期215-217,225,共4页Chinese Journal of Zoonoses

基　　金：浙江省自然科学基金(Y205700)

摘　　要：目的测定汉坦病毒Z5株M、S片段的全长序列,了解其分子结构特征,并分析Z5与其他汉坦病毒株的遗传学差异。方法设计引物,用RT-PCR技术扩增Z5株全长M与S片段。PCR产物纯化后克隆入T载体并测定序列及分析。结果Z5株M片段的全基因序列含有3 616个核苷酸,编码1 135个氨基酸。S全基因序列含有1 700个核苷酸,编码429个氨基酸。经核苷酸与氨基酸同源性分析,Z5株应属于汉坦病毒的汉滩型(HTN型)毒株,与Z10株同属一个亚型。结论Z5株病毒和其他汉滩病毒病毒在核苷酸序列上有差异,但在氨基酸水平上的同源性仍较高。The M and S segment cDNAs of hantavirus Z5 strain was amplified by RT-PCR,and the purified PCR products were cloned into vector pGEM-T and then sequenced.It was demonstrated that the M genome segment of Z5 was found to be 3 616 nucleotides in length with a single open reading frame encoding 1 135 amino acids.And the S genome segment was 1700 nucleotides in length with a single open reading frame encoding 429 amino acids.As demonstrated by the homologous analysis of nucleotides and amino acids,it was showed that the Z5 strain belonged to hantaan viruses HTN type and was the same subtype of the Z10 strain.It is conclouded that difference in nucleotide sequence exists between Z5 strain with other Hantavirus strains but high level of homology in amino acid sequences is still present.

关 键 词：汉坦病毒 M和S基因片段 序列分析 

分 类 号：R373.3[医药卫生—病原生物学]