汉防己甲素对益康唑抗须癣毛癣菌活性增效作用的体内实验研究  

An in vivo study on tetrandrine as a synergist to econazole against Trichophyton mentagrophytes

作　　者：张丁[1] 张宏[1] 李海瑞[2] 李水秀[2] 石建萍[1,3] 

机构地区：[1]暨南大学附属第一医院皮肤科,广州510632 [2]暨南大学国际学院 [3]广州医学院附属深圳沙井医院皮肤科

出　　处：《中国人兽共患病学报》2010年第3期222-225,共4页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(30972660);广东省科技计划项目(2008B030301350)

摘　　要：目的探讨体内汉防己甲素(tetrandrine,TET)对益康唑(econazole,ECZ)抗须癣毛癣菌活性的增效作用。方法以须癣毛癣菌构建豚鼠皮肤癣菌病模型,将造模成功的40只豚鼠随机分为4组,分别涂用1%ECZ+1%TET(ECZ+TET组)、1%ECZ(ECZ组)、1%TET(TET组)和霜剂基质(基质组)每d 1次,连续14d,从皮损一般情况、病原学、病理学三方面观察TET对ECZ抗须癣毛癣菌的增效活性。结果ECZ+TET组从用药第4d起,皮损评分开始呈持续下降趋势;用药第14d的皮损评分降至0.75分。ECZ组皮损评分与之类似,但总体下降趋势小于ECZ+TET组,用药第14d的皮损评分为2.00分。两组皮损评分差异有统计学意义(P=0.037)。ECZ+TET组皮损组织真菌培养阳性率、病理组织中真菌菌丝和孢子、炎症反应和组织破坏程度明显轻于其它3组。结论TET在须癣毛癣菌所致豚鼠皮肤癣菌病模型中能增加ECZ抗须癣毛癣菌活性。To investigate the synergistic effect of tetrandrine（TET） to the anti-Trichophyton mentagrophytes activity of econazole（ECZ） in vivo,animal model of dermatophytosis in guinea pig was established by inoculating Trichophyton mentagrophytes conidia.These 40 infected animals were divided into 4 groups randomly and from Day 5 after inoculation,they were treated with the following formulations once per day for successive 14 days： 1% econazole and 1% tetrandrine compound cream（1%ECZ＋1%TET group）,1% econazole cream（1%ECZ group）,1%TET cream（1%TET group） and matrix cream（matrix group）.The alterations in the skin lesions and the pathological changes were observed.From the 4th day of drugs applying,the tendency of lesion score about 1%ECZ＋1%TET group continued to decline.On the 14th day of drugs applying,the lesion score decreased to 0.75.There was a similarity between 1%ECZ＋1%TET group and 1%ECZ group in tendency of lesion score,but more reduction was appeared in 1%ECZ＋1%TET group than in 1%ECZ group（mean 0.75 VS 2.00） with obviously statistical difference（P=0.037）.The culture results of skin sections and the pathological changes in 1%ECZ＋1%TET group were less than those of other 3 groups.It＇s predicted that there is a synergistic effect of tetrandrine to the anti-Trichophyton mentagrophytes of econazole in animal model of dermatophytosis in guinea pig.

关 键 词：汉防己甲素 益康唑 增效作用 皮肤癣菌病 

分 类 号：R699.2[医药卫生—泌尿科学]