新基因AY358935的生物信息学分析及功能预测  

Bioinformatics analysis and function prediction of the novel gene AY358935

作　　者：熊绍权[1] 杨寒朔[2] 龙奇达[3] 

机构地区：[1]广州中医药大学第一附属医院肿瘤科,广东广州510405 [2]四川大学生物治疗国家重点实验室,四川成都610041 [3]广州市妇婴医院妇产科,广东广州510180

出　　处：《南方医科大学学报》2010年第2期232-235,238,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(30700699)

摘　　要：目的对新基因AY358935的功能性质进行生物信息分析,为深入研究该基因功能奠定基础。方法利用生物信息学分析软件,分析新基因AY358935及其编码蛋白的性质、定位、结构、表达谱等特征,并根据相关信息预测其生物学功能。利用Western blotting检测病毒感染早期AY358935的表达变化。结果AY358935基因进化保守,与马、小鼠、斑马鱼、非洲爪蟾的相似性分别为74%、60%、38%、33%。亚细胞定位于线粒体的可能性大。含有一个N末端信号肽序列和单次跨膜结构,多个磷酸化位点,二级结构主要是α螺旋和无规则卷曲。在正常组织和癌组织表达广泛,并受双链RNA依赖蛋白激酶的表达调控。AY358935蛋白表达在水泡性口炎病毒感染2h后即明显上调。结论初步预测AY358935为具有重要功能的小分子分泌蛋白,可能参与细胞增殖和抗病毒天然免疫调控。Objective To obtain the functional information of AY358935 gene.Methods The properties,subcellular location,and structure of AY358935 protein,and the expression profile of AY358935 gene were analyzed by bioinformatics software and the biological functions of the gene were predicted.AY358935 expression was detected by Western blot analysis in early virus infection.Results AY358935 was evolutionally conserved.The human AY358935 protein had an amino acid similarity of 74%,60%,38% and 33% with its counterpart in horses,mice,zebrafish and Xenopus laevis,respectively.Bioinformatics analysis indicated that AY358935 protein was located likely in the mitochondria.There was a N-terminal signal peptide and single transmembrane structure in AY358935 protein,which contained several phosphorylation sites.The secondary structure mainly comprised of alpha helices and random coils.AY358935 was ubiquitously expressed in normal tissues and carcinomas and regulated by the expression of double-stranded RNA-dependent protein kinase.AY358935 protein expression was obviously upregulated in cells 2 h after infection by vesicular stomatitis virus.Conclusion As a predicted secretary protein with a small molecular weight,AY358935 might have important functions in cellular proliferation and anti-viral innate immune regulation.

关 键 词：AY358935 生物信息学 分泌蛋白 双链RNA依赖蛋白激酶 水泡性口炎病毒 

分 类 号：Q78[生物学—分子生物学]