超声造影对心肌毛细血管通透性的影响  

Impact of ultrasound-mediated microbubbles on myocardial vascular permeability in rats

作　　者：周冰洁[1] 查道刚[1] 杜荣生[1] 胡锋[1] 吴爵非[1] 刘伊丽[1] 

机构地区：[1]南方医科大学南方医院心内科,广东广州510515

出　　处：《南方医科大学学报》2010年第2期239-241,共3页Journal of Southern Medical University

基　　金：国家自然科学基金(30870721)

摘　　要：目的研究高机械指数、高剂量微泡造影剂条件下超声造影对心肌毛细血管通透性的影响并观察其恢复正常的时间。方法将30只Wistar雄性大鼠随机分为5组。A～D组采用相同的超声造影条件,但各组伊文思蓝(EB)注射时间不同:分别为超声造影前10s、造影结束即刻、造影结束后5min、造影结束后20min给予EB;E组为对照组(假造影组),即5min超声照射结束后即刻再注射造影剂微泡及EB。所有动物均在EB注射后5min处死。测定心脏EB渗出面积百分比及单位心肌内EB含量。结果A、B、C组EB渗出面积明显高于对照组(P<0.05),D组渗出面积与对照组无差异(P>0.05);A、B组心肌EB含量明显高于对照组(P<0.05),C及D组心肌EB含量与对照组无差异(P>0.05)。各组造影前后心率及心律失常数目无差异(P>0.05)。结论高机械指数超声联合高剂量微泡造影剂可使心肌毛细血管通透性增加,其恢复时间在超声造影结束后20min内。Objective To investigate the impact of high-dose microbubbles induced by high mechanical index myocardial contrast echocardiography（MCE） on vascular permeability and its recovery time in rats.Methods Thirty male Wistar rats were randomized into 4 MCE groups（groups A-D） and a control group.In the MCE groups,Evans blue was injected at 10 s before MCE（A）,immediately after the end of MCE（B）,and at 5 min（C） and 20 min after the end of MCE（D）.In the control group,the microbubbles and Evans blue were injected at the end of a 5-min ultrasound exposure.All the rats were sacrificed 5 min after Evans blue injection,and the content of Evans blue in the myocardium and the percentage of Evans blue leakage area were determined.Results The percentage of Evans blue leakage area in groups A,B and C were significantly higher than that in the control group（P〈0.05）,while the percentage was similar between group D and the control group（P〉0.05）.Evans blue contents in groups A and B were significantly higher than that in the control group（P〈0.05）,but groups C and D showed comparable contents with the control group E（P〉0.05）.No significant changes of the heart rates and premature beat number were observed during and after MCE in these groups（P〉0.05）.Conclusion High mechanical index MCE and a high contrast dose may induce increased microvascular leakage in rats,and the vascular permeability can recover in 20 min after MCE.

关 键 词：超声 心肌声学造影 血管通透性 伊文思蓝 

分 类 号：R445.1[医药卫生—影像医学与核医学]