多沙普仑对新生大鼠延髓脑片呼吸节律性放电的调节作用  

Effect of doxapram on the respiratory rhythmical discharge activity in the brainstem slice of neonatal rats

作　　者：李国才[1] 焦勇钢[1] 吴中海[1] 方芳[1] 程静[1] 

机构地区：[1]南方医科大学生理学教研室,广东广州510515

出　　处：《南方医科大学学报》2010年第2期245-248,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30570670);广东省医学科研基金(A2008241)

摘　　要：目的观察多沙普仑对新生大鼠延髓脑片呼吸节律性放电(RRDA)的调节作用。方法制作主要包含延髓面神经后核内侧区(mNRF)的大鼠离体脑片标本,并保留舌下神经根的完整。将36只新生SD大鼠随机分为Ⅰ～Ⅵ组(n=6)。Ⅰ组为对照组;Ⅱ～Ⅳ组为多沙普仑组(浓度分别为2、5、10μmol/L),Ⅴ组给予丙泊酚(20μmol/L),Ⅵ组给予丙泊酚(20μmol/L)+多沙普仑(5μmol/L),观察给药后l、3、5、l0、l5、30min时舌下神经根呼吸节律性放电的变化,并分析吸气时程(TI)、呼气时程(TE)、呼吸周期(RC)、放电积分幅度(IA)的改变。结果Ⅱ组在给药前后脑片RRDA并无明显改变;Ⅲ、Ⅳ组在给药后1min开始,吸气时程明显延长,放电积分幅度增加;呼气时程于第5min时开始缩短(P<0.05),但呼吸周期仅于第10min时缩短,其余时间点无明显改变;Ⅴ组在第3min时开始出现吸气时程缩短、放电积分幅度下降,并伴有呼气时程及呼吸周期延长(P<0.05),第10min时达最大变化值;Ⅵ组RRDA各时间点均无明显改变(P>0.05)。结论5μmol/L多沙普仑可直接兴奋延髓脑片的RRDA,且可完全拮抗丙泊酚对脑片RRDA的抑制作用,此作用主要通过兴奋吸气中枢实现。Objective To investigate the effects of doxapram on the respiratory rhythmical discharge activity（RRDA） in the brainstem slices of neonatal rats.Methods Thirty neonatal SD rats（of either sex,0-3 days old） were randomly divided into 6 equal groups（groups I-VI）,and the brainstem slices which contained the medial region of the nucleus retrofacialis（mNRF） were prepared.All the slices were perfused with modified Kreb＇s solution（MKS）,and in group I（control group）,the slices were perfused with MKS only;in groups Ⅱ to IV,the slices were perfused with doxapram in MKS continuously at the concentrations of 2,5,and 10 μmol/L,respectively;in groups V and VI,the slices were perfused with 20 μmol/L propofol and 20 μmol/L propofol plus 5 μmol/L doxapram,respectively.The RRDA in the hypoglossal nerve was recorded by suction electrode.The discharge time course of the inspiratory（TI）,expiratory（TE）,respiratory cycle（RC） and integral amplitude of the inspiratory discharge（IA） were recorded at 1,3,5,10,15,and 30 min after the application of the drugs.Results The hypoglossal nerve in groups I,Ⅱ and VI showed no significant changes of RRDA in the entire course of the experiment（P〉0.05）.In groups Ⅲ and IV,the TI,IA increased and TE decreased significantly 5 min after doxapram application（P〈0.05）,and the RC was shortened only at 10 min.In group V,the TI and IA decreased and the RC and TE increased significantly after the drug application（P〈0.05）.Conclusion Doxapram（〉5 μmol/L） can directly stimulate the RRDA and prevent propofol-induced inhibitory effects in the brainstem slice of neonatal rats,and the effects are mediated by its actions upon the inspiratory neurons in the mNRF.

关 键 词：多沙普仑 面神经后核内侧区 呼吸节律性放电 延髓 

分 类 号：R965[医药卫生—药理学]