脂质体介导的HSV-tk基因转移对烫伤大鼠成纤维细胞凋亡的影响  

Effect of liposome-mediated HSV-tk gene transfer on fibroblast apoptosis in rats with scald injury

作　　者：杨磊[1] 高建华[2] 王甲汉[1] 

机构地区：[1]南方医科大学南方医院烧伤科,广东广州510515 [2]南方医科大学南方医院整形科,广东广州510515

出　　处：《南方医科大学学报》2010年第3期544-546,共3页Journal of Southern Medical University

基　　金：广东省自然科学基金(9151051501000067);南方医院院长基金(2008B024)

摘　　要：目的通过脂质体介导外源基因转导烧伤创面的手段,探讨单纯疱疹病毒胸苷激酶(HSV-tk)基因转移对创面成纤维细胞凋亡的影响。方法利用我们已经构建成功的重组真核表达质粒pcDNA3.1/HSV-tk,通过脂质体介导转染烫伤大鼠皮肤组织,用RT-PCR法检测HSV-tk基因的表达,在给与抗病毒药物丙氧鸟苷(GCV)后,用透射电镜观察成纤维细胞的凋亡情况。结果RT-PCR结果显示脂质体介导的tk基因转移可在创面成纤维细胞中出现阳性表达,给予GCV后可诱导表达tk基因的成纤维细胞出现凋亡。结论脂质体介导的HSV-tk基因转移可促进成纤维细胞凋亡。Objective To evaluate the effect of HSV-tk gene transfer on the apoptosis of fibroblasta in rats with scald injury. Methods The recombinant eukaryotic expression vector pcDNA3.1/tk was transfected via liposome in the skin of rats with scald injury. The expression of tk gene was detected by RT-PCR technique, and after GCV injection, the apoptosis of the fibroblasts positive for tk gene was observed under transmission electron microscope. Results Liposome-mediated HSV-tk gene transfection of the rat skin resulted in the positive expression of tk gene in the fibroblasts in the burn wound. GCV injection induced the apoptosis of the positively transfected fibroblasts. Conclusion HSV-tk gene transfer mediated by liposome can promote the apoptosis of the fibroblasts in rats with scald injury.

关 键 词：基因治疗 成纤维细胞 凋亡 HSV-TK 

分 类 号：Q343[生物学—遗传学]