微生物浊度法与管碟法测定乙酰吉他霉素颗粒剂的含量比较  

The Comparision of the Content Determination of Acetylkitasamycin Granules by Turbidimetric Method and Agar Diffusion Method

作　　者：高燕霞[1] 姜建国[1] 韩彬[1] 

机构地区：[1]河北省药品检验所,石家庄050011

出　　处：《中国药品标准》2010年第1期56-58,共3页Drug Standards of China

摘　　要：目的:建立浊度法测定乙酰吉他霉素颗粒剂的方法,并与管碟法进行比较。方法:浊度法以金黄色葡萄球菌为试验菌,加菌量2.0%～2.5%(V/V),(37±0.5)℃培养4～5hr左右测定。结果:浊度法抗生素线性浓度为0.45～6.3IU.mL-1。与管碟法测定含量比较,无显著性差异。结论:浊度法灵敏,快速,影响因素较少,与管碟法均可作为测定乙酰吉他霉素颗粒剂效价的方法并存。Objective： To establish a turbidimetric method for the content determination of Acetylkitasamycin Granules, compared with agar diffusion method. Method： The test organism was Staphylococcus aureus and cultured with fresh medium （pH 7.0）. Adjust spore suspension of Staphylococcus aureus inoculate with a 2. 0% -2. 5% （V/V）ratio at （37±0.5）℃ for about 4 -5 hr. Results： The linear range of Acetylkitasamycin was 0. 45 - 6. 3 IU · mL^ -1. And the contents of two methods had no significant difference. Conclusion： The turbidimetric method was sensitive, rapid and feasible to determine Acetylkitasamycin Granules, it can concomitance with agar diffusion method.

关 键 词：效价 浊度法 管碟法 乙酰吉他霉素颗粒剂 

分 类 号：R978.15[医药卫生—药品]