黄连解毒汤70%醇提物对MDR模型小鼠S180肿瘤细胞凋亡因子表达的影响  

Influence of 70% ethanol extract from Huanglian Jiedu Decoction on expression of S180 tumor cell apoptosis factor in MDR model mice

作　　者：孙付军[1] 宋卫国[1] 李贵海[1] 杨书斌[1] 

机构地区：[1]山东省中医药研究院药理研究室,济南250014

出　　处：《中华中医药杂志》2010年第4期610-612,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：山东省自然科学基金资助项目(No.Y2005C57)~~

摘　　要：目的:观察黄连解毒汤70%醇提物对MDR模型小鼠S180肉瘤细胞凋亡因子的影响,探讨其逆转多药耐药作用的分子机制。方法:模拟临床PFC方案,建立小鼠S180肉瘤细胞多药耐药在体模型,同时给予黄连解毒汤70%醇提物灌胃10d,流式细胞仪荧光检测观察细胞凋亡因子Fas、Trail、细胞黏附因子CD54等指标。结果:黄连解毒汤70%醇提物增加耐药细胞凋亡因子Fas、Trail表达率,促进耐药S180细胞的凋亡,降低细胞黏附因子CD54的表达。结论:黄连解毒汤70%醇提物促进耐药细胞凋亡因子的高表达,是其逆转肿瘤多药耐药的途径之一。Objective： To observe the effect of 70% ethanol extract from Huanglian Jiedu Decoction on expression of S180 tumor cell apoptosis factor in MDR model mice, and to research the molecule biology base fot directing clinic. Methods： simulate Clinic PFC project was simulated, the mice model of multi-drug resistance of S180 tumour cell was established. 70% Ethanol extract from Huanglian Jiedu Decoction were given for 10 days. The flow cytometry was used to observe Fas, Trail, CD54.,et al. Resluts： 70% Ethanol extract from Huanglian Jiedu Decoction can increase expression rate of Fas, Trail and improve apoptosis of S180 cell, at the same time it can decrease expression of CD54.Conclusions： 70% Ethanol extract from Huanglian Jiedu Decoction improve high level expression of apoptosis factor, and it maybe one of pass reverse multi-drug resistance of tumour.

关 键 词：黄连解毒汤%醇提物 肿瘤多药耐药 小鼠 Fas TRAIL CD 凋亡因子 黏附因子 耐药细胞 模型组 

分 类 号：R285.5[医药卫生—中药学]