复方苁蓉益智胶囊对痴呆老龄大鼠海马MARCKS mRNA表达的影响  

Effect of Fufang Congrong Yizhi Capsule on myristoylated alanine-rich C-kinase substrate (MARCKS) mRNA level in hippocampus of old dementia rats

作　　者：苏芮[1] 韩振蕴 范吉平 

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]北京市医药大学东方医院,北京100078 [3]中田中医科学院,北京100700

出　　处：《中华中医药杂志》2010年第4期620-622,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.30701137)~~

摘　　要：目的:研究复方苁蓉益智胶囊对β-淀粉样蛋白(1-40)致痴呆老龄大鼠海马MARCKS mRNA表达的影响。方法:将大鼠随机分为年轻组、老龄组、假手术组、模型组、中药组和西药组,对老龄大鼠采用β-淀粉样蛋白(1-40)一次性侧脑室注射,建立痴呆模型,中药和西药组分别予复方苁蓉益智胶囊和安理申溶液灌胃14d。通过水迷宫以及RT-PCR检测,观察大鼠学习记忆功能以及MARCKS mRNA的变化。结果:行为学检测表明中药组和西药组的学习记忆功能明显高于模型组,模型组海马MARCKS mRNA比正常组显著增高,而中药组海马MARCKS mRNA比模型组明显降低。结论:复方苁蓉益智胶囊能够降低脑室注射β-淀粉样蛋白(1-40)致痴呆老龄大鼠海马MARCKSmRNA的表达,并改善痴呆老龄大鼠学习记忆功能。Objective：To observe the effect of Fufang Congrong Yizhi Capsule on myristoylated alanine-rich C-kinase substrate （MARCKS） mRNA level in hippocampus of old dementia rats induced by β-amyloid（1-40）. Methods： Animals were divided randomly into young group, old group, sham operation group, model group, western medicine group and Chinese medicine group. Model, western and Chinese medicine groups received β-amyloid （1-40） intracerebroventrical injection to build dementia animal model, and then Chinese and westein medicine groups received Fufang Congrong Yizhi Capsule and donepezil for 14 days, respectively. To observe changes of behavior and MARCKS mRNA under the effect of Chinese and western medicine by Morris test and RT-PCR test. Results： Learning and memory function of Chinese and western medicine groups were better than that of model group. MARCKS mRNA of model group was increased significantly, that of Chinese medicine group was decreased compared with model group. Conclusion： Fufang Congrong Yizhi Capsule can reduce the level of MARCKS mRNA in hippocampus of old dementia rats caused by β-amyloid（1-40）, through which it can improve the learning and memory function of the dementia old rats.

关 键 词：老年性痴呆 β淀粉样蛋白(-) 老龄大鼠 MARCKS mRNA 神经元可塑性 益智胶囊 模型组 学习记忆功能 海马 正常组 

分 类 号：R285.5[医药卫生—中药学]