高效液相色谱法测定酒豨莶草中奇壬醇的含量  

Determination of kirenol in Herba Siegesbeckiae Preparata by high performance liquid chromatography

作　　者：刘赞[1] 侴桂新 王峥涛[1,2] 

机构地区：[1]中国药科大学,江苏南京210009 [2]上海中药标准化研究中心,上海201203

出　　处：《中国中药杂志》2010年第6期729-731,共3页China Journal of Chinese Materia Medica

基　　金：《中国药典》2010年版一部标准研究课题任务书(YS-178);上海市科学技术委员会中药现代化专项课题(08DZ1970103)

摘　　要：目的：建立测定酒稀莶草中奇壬醇含量的高效液相色谱方法。方法：BostonCrestODS色谱柱（4．6mm×250mm，5μm）；以乙腈为流动相A，以水为流动相B，进行梯度洗脱；检测波长215nm，流速1．0mL·min。结果：奇壬醇在0．0202～20．02¨g呈良好的线性关系（r=0．9998），该方法平均回收率为99．62％，RSD2．0％。结论：该方法简便易行、准确、重复性好，可用于酒稀莶草中奇壬醇的含量测定。Objective ： To establish a high performance liquid chromatographic method for the determination of kirenol in Herba Siegesbeckiae Praeparata. Method： The analysis was carried out on a Boston Crest ODS column （4. 6 mm×250mm,5μm） with gradient elution using acetonitrile-water as mobile phases. The flow rate was 1.0 mL · min^-1l and the detection wavelength was at 215 nm. Result ： The calibration curve was linear over the range of 0. 020 2-20. 02 μg for kirenol. The correlation coefficient of the calibration crave was 0. 999 75. The average recovery was 99. 62% with relative standard derivation （RSD） of 2. 0%. Conclusion： The results showed that the method is simple, accurate and repeatable and it is suitable for the determination of kirenol in Herba Siegesbeckiae Praeparata.

关 键 词：酒稀莶草 奇壬醇 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]