毒热平注射液对巨噬细胞TIR信号通路的影响  

Effect of Dureping injection on TIR signal pathway on Ana-1 cells

作　　者：张艳丽[1] 王宁萍[1] 顾立刚[2] 李澎涛[2] 

机构地区：[1]宁夏医科大学病原生物学与免疫学教研室,宁夏银川750004 [2]北京中医药大学中医药抗病毒教育部重点实验室,北京100029

出　　处：《中国中药杂志》2010年第6期741-744,共4页China Journal of Chinese Materia Medica

基　　金：宁夏医科大学特殊人才启动项目(XT200802)

摘　　要：目的:研究毒热平注射液对流感病毒感染的小鼠腹腔巨噬细胞株Ana-1细胞TIR(Toll/IL-1 receptors)信号传导通路的影响。方法:用100 TCID50流感病毒亚甲型鼠肺适应株A/FM/1/47(H1N1)感染小鼠腹腔巨噬细胞株Ana-1后换用10,1 mg.L-1含药维持液继续培养,分别于12,24 h弃细胞上清液并收集细胞,提取巨噬细胞RNA,进行实时定量PCR反应(RT-PCR),动态测定毒热平注射液对病毒感染前后巨噬细胞TIR信号传导通路中各信号蛋白:Toll样受体7(TLR7)、髓样分化因子88(MyD88),IL-1受体相关激酶4(IRAK4)、肿瘤坏死因子相关激酶6(TRAF6)和核因子κB(NF-κB)mRNA表达水平的影响。结果:毒热平注射液可剂量依赖性地下调病毒感染巨噬细胞TLR7,MyD88,IRAK4,TRAF6,NF-κB mRNA的表达水平。结论:毒热平注射液能通过调节TIR信号传导通路各信号蛋白的活性发挥抗病毒感染作用。Objective： To investigate the influence of Dureping injection to the murinal celiac macrophage Ana-1 on TIR signal pathway. Method： Ana-1 cell line was infected by influenza virus FM1 strain and treated with the Dureping injection in different con- centrations （10,1 mg · L^-1 group） for 12 h and 24 h. Then we collected the cells, extracted mRNA and measured the expressions of TLR7, MyD88, IRAK4, TRAF6 and NF-κB p65 respectively by RT-PCR. Result： Dureping injection down-regulated the expression of TLR7 ,MyD88, IRAK4, TRAF6 and NF-κB p65 mRNA in Ana-1 cell line infected by influenza virus, in a dose-dependent manner significantly. Conclusion： Dureping injection has an obvious effect against influenza virus FM1 strain by regulating the TIR signal pathway.

关 键 词：Ana一1 毒热平注射液 流感病毒 TIR TLR7 

分 类 号：R285.5[医药卫生—中药学]