废次烟叶中绿原酸的提取与分离  

Extraction and Separation of Chlorogenic Acid from Waste and Sub-standard Tobacco

作　　者：古君平[1] 魏万之[1] 

机构地区：[1]湖南大学化学生物传感与计量学国家重点实验室,长沙市岳麓区麓山南路2号410082

出　　处：《烟草科技》2010年第2期43-47,共5页Tobacco Science & Technology

摘　　要：考察了提取溶剂种类、乙醇浓度、料液比、提取时间、提取溶剂pH值,以及HPD-400大孔树脂吸附与脱附条件对废次烟叶中绿原酸提取与分离的影响。结果表明:①超声辅助提取的适宜条件为:提取溶剂30%(pH4)乙醇,料液比1∶10,提取时间45min;②烟叶绿原酸提取液的层析条件为:层析柱:HPD-400大孔树脂柱;上柱液浓度4.0mg/mL;上柱液体积:42.5mL;流速1.42mL/min;洗脱液191.7mL30%乙醇水溶液。采用此条件,10.1g废次烟叶样品可提取0.335g固形物,其绿原酸含量为31.9%,绿原酸提取率为1.71%,层析回收率72.3%。The effects of experimental conditions,such as extracting solvent,alcohol concentration,ratio of material to liquid,extracting time,pH value of extracting solvent,and condition of adsorption-desorption by HPD-400 macroporous resin,on the extraction and separation of chlorogenic acid from waste and sub-standard tobacco were investigated.The results showed that：1）the appropriate conditions of extraction assisted by ultrasonic were extracting solvent 30%（pH 4）ethanol,ratio of material to liquor 1∶10,extraction time 45 minutes;2）the chromatographic conditions for the extracted solution of chlorogenic acid were HPD-400 macroporous resin column;42.5 mL of extracted chlorogenic acid solution at a concentration of 4.0 mg/mL,flow rate 1.42 mL/min;elution solution 191.7 mL 30% ethanol.Under the said conditions,0.335 g solid material containing chlorogenic acid of 31.9% was obtained from 10.1 g of tobacco,the yield of chlorogenic acid was 1.71% and the chromatographic recovery of chlorogenic acid reached 72.3%.

关 键 词：废次烟叶 绿原酸 HPD-400大孔树脂 

分 类 号：TS411.1[农业科学—烟草工业]