黄芪甲甙干预小鼠骨髓间充质干细胞凋亡的研究  

Effects of astrugaloside on the apoptosis of rat bone marrow mesenchymal stem cells induced by adriamycin

作　　者：刘丹莉[1] 杨云华[1] 于小华[1] 李双杰[1] 

机构地区：[1]南华大学附属第一医院,衡阳421001

出　　处：《陕西医学杂志》2010年第3期259-262,共4页Shaanxi Medical Journal

基　　金：国家自然科研基金资助项目(30271665);湖南省教育厅科研基金资助项目(C5C458)

摘　　要：目的:观察黄芪甲甙(AST)对小鼠骨髓间充质干细胞(BMSCs)凋亡的影响。方法:从Balb/c小鼠股骨骨髓中提取BMSCs并鉴定,加入阿霉素(ADR)(10μg/L)诱导BMSCs凋亡,同时加入不同剂量的MR与BMSCs共培养24h,实验共分6组,即空白对照组,ADR组、ADR+AST(4μg/L、40μg/L、400μg/L)组、MR400μg/L组。采用TUNEL法和流式细胞仪检测细胞凋亡的情况。结果:TUNEL和流式结果表明黄芪甲甙有抑制BMSCs凋亡的作用,40μg/L组作用较强,与ADR组比较差异有显著性,增加浓度抑制凋亡的作用增加不明显,ADR+AST(40μg/L、400μg/L)两组,细胞凋亡率差异无显著性。结论:黄芪甲甙具有抑制阿霉素诱导的骨髓间充质干细胞凋亡的作用,无剂量依赖性。Objective：To determine the effect of astragaloside on the apoptosis of mesenchymal stem cells（BMSCs）induced by adriarnycinin vitro.Methods：From Balb/c mouse femur bone marrow extracted and identified BMSCs adding ADR（Adriamycin,ADR）（10μg/L）induced apoptosis BMSCs at the same time with different doses of BMSCs with AST co-culture 24h,the trial is divided into six groups,That is,the control group,ADR group,ADR ＋ AST（4μg/L,40μg/L,400μg/L）group,MR400μg/L group.TUNELL using flow cytometry and apoptosis.Results：TUNEL-flow and the results have shown that astragalus BMSCs inhibit apoptosis,40μg/L the role of a strong group,compared with the ADR silk there was a significant difference,to increase the role of apoptosis inhibitory concentration was not obvious to increase,ADR ＋ AST（40μg/L,400μg/L）two groups,the rate of apoptosis was no significant difference.Conclusion：Astragalus with adriamycin-induced suppression of bone marrow mesenchymal stem cells apoptosis,and no dose-dependent.

关 键 词：细胞凋亡/预防和控制 @黄芪甲甙 干细胞 骨髓/分析 干预性研究 小鼠 

分 类 号：R973[医药卫生—药品]