乌头类中药中药效指标成分的确定及含量测定  

Content Determination of Medicinal Components of Aconitum carmichaeli

作　　者：刘永刚[1] 刘倩[2] 张宏桂[1] 乔延江[1] 

机构地区：[1]北京中医药大学中药学院,北京市100102 [2]华北煤炭医学院,唐山市060031

出　　处：《中国药房》2010年第11期1002-1003,共2页China Pharmacy

基　　金：国家自然科学基金资助课题(30901959)

摘　　要：目的:确定乌头类中药中药效指标成分并测定其含量。方法:初步确定新乌头次碱可作为乌头类中药的药效指标成分。采用高效液相色谱法测定新乌头次碱的含量:色谱柱为AgilentZobaxXDBC1(8250mm×2.1mm,5μm),流动相为0.01%氨水溶液-乙腈(梯度洗脱),流速为1mL·min-1,检测波长为240nm。结果:新乌头次碱进样量在0.51～10.2μg范围内与峰面积积分值呈良好线性关系(r=0.9998);平均回收率为102.1%,RSD=2.2%(n=6)。结论:本方法简便、准确、重复性好,可用于乌头类中药的质量控制。OBJECTIVE： To establish the method for the content determination of medicinal components in Aconitum carmichaeli. METHODS： Benzoylmesaconine was preliminary considered as medicinal components of A. carmichaeli. HPLC was applied to determine the content of benzoylmesaconine and performed on Agilent Zobax XDB C18 column （250 mm×2.1 mm, 5 um）. Mobile phase consisted of 0.01% ammonia-acetonitrile （gradient elution） with flow rate of 1 mL. min^-1 and the detection wavelength were set at 240 nm. RESULTS： The linear ranges of benzoylmesaconine was 0.51-10.2 ug. The average recovery of benzoylmesaconine was 102.1% （RSD=2.2%, n=6）. CONCLUSION： The method is simple, accurate and reproducible for the quality control ofA. carmichaeli.

关 键 词：质量控制 乌头类中药 新乌头次碱 药效指标 毒性指标 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]