酮通颗粒质量标准研究  

Quality Standard of Tongtong Granule

作　　者：余晓东[1] 武夏明[2] 刘田云[1] 赵广成[2] 刘逢芹[1] 

机构地区：[1]山东大学附属千佛山医院,济南市250014 [2]山东大学药学院,济南市250012

出　　处：《中国药房》2010年第11期1025-1027,共3页China Pharmacy

摘　　要：目的:建立酮通颗粒的质量标准。方法:采用薄层色谱(TLC)法鉴别制剂中三七、银杏叶、葛根药材;用高效液相色谱(HPLC)法测定制剂中银杏总黄酮醇苷的含量。结果:TLC斑点清楚,专属性强。槲皮素、山柰素、异鼠李素的检测浓度分别在5.24～83.84、4.56～72.96、2.48～39.68μg·mL-1范围内与各自峰面积积分值呈良好线性关系(r分别为0.9992、0.9991、0.9994);三者平均回收率分别为96.21%、99.14%、95.00%,RSD分别为2.06%、1.19%、1.31%。结论:所建标准可用于酮通颗粒的质量控制。OBJECTIVE： To establish the quality standard of Tongtong granule. METHODS： Panax notoginseng, Ginkgo biloba and Pueraria lobata were identified qualitatively by TLC and the content of total flavonol glyconsides in G. biloba was determined by HPLC. RESULTS： The TLC spots were distinctive and specific. The linear range of quercetin, kaempferide, isorhamnetin were 5.24- 83.84ug·mL^-1 （r=0.999 2）, 4.56-72.96ug·mL^-1 （r=0.999 1） and 2.48-39.68 ug·mL^-1 （r=0.999 4）, respectively. The average recoveries were 96.21% （RSD = 2.06 % ）, 99.14 % （RSD = 1.19 % ） , 95.00 % （RSD = 1.31% ）, respectively. CONCLUSION ： The method is reliable, accurate, and reproducible for the quality control of Tongtong granule.

关 键 词：酮通颗粒 薄层色谱法 高效液相色谱法 总黄酮醇苷 三七 葛根 

分 类 号：R283.627[医药卫生—中药学] R927.1[医药卫生—中医学]