不同证型缓解期哮喘儿童的T细胞亚群状态初探  

Research of Significance of T-lymphocyte Subsets in Paracmasis of Children with Different Asthmatic Type

作　　者：冯金环[1] 秦璞[1] 王霖[1] 

机构地区：[1]山东省青岛市海慈医疗集团海慈医院,青岛266033

出　　处：《中国中医急症》2010年第3期446-448,共3页Journal of Emergency in Traditional Chinese Medicine

摘　　要：目的探讨缓解期哮喘儿童肺虚、脾虚、肾虚型外周血T细胞亚群状态及意义。方法设对照组(n=38)及哮喘组(n=54),哮喘组根据中医辨证分为肺虚型组(n=15)、脾虚型组(n=17)和肾虚型组(n=22)。采用单克隆抗体间接免疫荧光法检测CD3+、CD4+、CD8+,统计检测结果并进行统计分析。结果哮喘组CD4+及CD4+/CD8+明显低于对照组,CD8+明显高于对照组。CD3+与对照组比较差异无统计学意义。肺虚型、脾虚型、肾虚型之间CD3+差异无统计学意义。肺虚型与脾虚型CD4+/CD8+均明显低于对照组及肾虚型,但以肺虚型为甚;两型之间CD4+、CD8+差异无统计学意义,但与对照组及肾虚型差异有统计学意义。肾虚型CD4+、CD8+与对照组比较差异无统计学意义,CD4+/CD8+低于对照组。结论T细胞亚群的紊乱程度以肺虚型最重,其次为脾虚型,再次为肾虚型。Objective： To investigate the significance of T-lymphocyte subsets in peripheral blood of paracmasis asthmatic children with lung deficiency （LD）, spleen deficiency （SD） and kidney deficiency （KD） . Methods： All children were divided into the control group and treatment group. In the treatment group, asthmatic children were divided into three groups of LD, SD and KD according to the relevant diagnosis standards. T cells of CD＋3, CD4＋, CD8＋ were measured by monoclonal antibody indirect immunofluorescence. The data was compared and took statistical analysis. Results： CD ＋4 cells and CD ＋4/CD ＋8 ratio in the treatment group were makedly lower. CD ＋s was higher and CD ＋3 had no significant difference than those in the control group. CD ＋3 had no difference among LD, SD, KD groups. CD ＋4/ CD ＋8 ratio of LD and SD groups were much lower than that in KD and control groups. The LD group was the lowest, but there was no significant difference between LD and SD groups. CD＋4/CD ＋8 ratio of KD group was lower than that in control group, but CD ＋4 and CD ＋8 had no significant difference between them. Conclusion： The most serious disorder in the T-lymphocyte subsets is occurred in the LD asthmatic children, followed by SD, again KD.

关 键 词：哮喘 T细胞亚群肺虚型 脾虚型 肾虚型 儿童 缓解期 

分 类 号：R725.6[医药卫生—儿科]