高世代转P_(SAG12)-IPT基因水稻生育后期的延衰性  

Study on senescence delay characteristics of transgenic rice containing anti-senescence chimeric gene P_(SAG12)-IPT in high generation at later developmental stages

作　　者：许明[1,2] 黄昕[2] 郑华坤[2] 苏永昌[2] 郑祥正[2] 程祖锌[1,2] 郑金贵 

机构地区：[1]福建农林大学作物遗传育种与综合利用教育部重点实验室 [2]福建农林大学农产品品质研究所,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2010年第1期1-5,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省科技创新平台建设项目(2007S1001);福建省科技厅重大专项专题项目(2008NZ0001-4);福建省教育厅科技项目(JA07063);福建农林大学青年教师科研基金(05B07)资助

摘　　要：以转PASG12-IPT基因水稻东南201自交高世代(T8)株系为材料,对始穗期和蜡熟期剑叶的衰老相关指标测定表明,蜡熟期高世代转PSAG12-IPT基因水稻株系的细胞分裂素含量、叶绿素含量、净光合速率值、CAT活性、SOD活性均显著高于受体品种,部分株系达到极显著水平,而转化株系的MDA含量则明显低于受体品种,POD活性呈无规律变化.由上可见,抑制衰老嵌合基因PSAG12-IPT对水稻叶片的衰老有一定的延缓效应,并能在高世代中稳定表达.The flag leaf-senescence related indexes of self-bred lines of transgenic rice ＇Dongnan201＇ containing anti-senescence chimeric gene PSAG12-IPT in high generation（T8） were determined during initial heading and dough stage.The results showed that the content of cytokinin,chlorophyll,photosynthetic rate,SOD and CAT activity of the high generation transgenic rice were all higher than that of the control and some transgenic lines reached very significant level,while the content of MDA was on the contrary.The POD activity changed irregularly during dough stage.This indicated that anti-senescence chimeric gene PSAG12-IPT has some delay effect on rice leaf-senescence and has expressed stably in high generation.

关 键 词：PSAG12-IPT基因 转基因水稻 叶片衰老 高世代 

分 类 号：S511[农业科学—作物学] S311