可降解组织工程心肌材料聚氨酯的体外评价  

In vitro evaluation of biodegradable cardiac tissue engineering polyurethane scaffold

作　　者：隋润钤[1,2] 韩健[3] 周建业[1] 胡盛寿[1] 周新民[2] 冯增国[3] 

机构地区：[1]中国医学科学院阜外心血管病医院,卫生部心血管再生医学重点实验室,北京市100037 [2]中南大学湘雅二医院心胸外科,湖南省长沙市410011 [3]北京理工大学材料科学与工程学院,北京市100081

出　　处：《中国组织工程研究与临床康复》2010年第8期1345-1348,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助(30772147)~~

摘　　要：背景：体外构建组织工程化心肌是当今医学领域的研究热点,支架材料的选择和设计是心肌组织工程的关键环节,但目前仍未找到理想的心肌支架材料。目的：对新型可降解材料聚氨酯进行体外评价,初步探讨其作为心肌组织工程支架的可行性。方法：以赖氨酸基二异氰酸酯为硬段,以赖氨酸为扩展链合成新型聚氨酯（PU-Lys）。在拉力机上测试材料的缝合强度和拉伸强度;在37℃,pH=7.4的磷酸盐缓冲溶液中检测聚氨酯的降解性能;采用细胞培养MTT法、细胞形态学观察方法,分析该聚氨酯的细胞毒性。结果与结论：力学性能检测得出该聚氨酯的拉伸强度为（8.1±0.1）MPa,缝合强度为（12.2±0.8）N;体外降解8周后质量损失为（13.1±0.3）%;MTT比色法结果显示细胞毒性为0~1级;细胞形态学观察显示L929细胞在聚氨酯材料浸提液中呈梭形或三角形,贴壁良好。提示此种新型解聚氨酯具有良好的力学性能和降解性,细胞相容性良好,符合组织工程心肌支架材料的应用要求。BACKGROUND：In vitro construction of tissue engineered cardiac muscle becomes a hot spot in recent years,and the selection and design of scaffold is the key link.However,there is lack of ideal cardiac tissue engineering scaffold material.OBJECTIVE：To evaluate the novel biodegradable polyurethane in vitro,and to discuss the feasibility of polyurethane as cardiac tissue engineeru scaffold.METHODS：A new type polyurethane（PV-Lys）was synthesized using diphenylmethane-4,4＇-diisocyanate as hard segment and lysine as expand chain.The tensile and suture strength were tested in vitro respectively,hydrolytic degradation was carried out in phosphate buffer saline of pH 7.4 at 37 ℃,and cytotoxicity was evaluated by MTT measurement and morphological observation.RESULTS AND CONCLUSION：The tensile strength of the polyurethane was up to（8.1±0.1）MPa,and the suture strength was（12.2±0.8）N.The average value of the mass loss of PV-Lys was（13.1±0.3）% at 8 weeks of in vitro hydrolytic degradation.MTT assay results showed that the cytotoxic grade of the novel PV-Lys was 0-1.Cell morphology observation showed that the L929 cells were spindle-shaped or triangular with good stretch.This PV-Lys scaffold is with favorable mechanical property,cytocompatibility,biodegradable property,which meets the requirements of tissue engineering application.

关 键 词：聚氨酯 生物医学工程 细胞相容性 水解降解 心肌支架材料 

分 类 号：R318[医药卫生—生物医学工程]