大型海藻多糖-琼脂糖改性敷料在动物皮肤再生中的作用  

Application of seaweed polysaccharide-agarose dressing to animal skin regeneration

作　　者：包磊[1] 黄建艳[1] 张灵敏[1] 储彬[1] 汤顺清[1] 

机构地区：[1]暨南大学生物医学工程研究所,广东省广州市510632

出　　处：《中国组织工程研究与临床康复》2010年第8期1369-1371,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：863计划项目资助(2007AA09Z436);广东省教育厅生物材料重点实验室开放基金资助(23607007)~~

摘　　要：背景:已经证实大型海藻多糖-琼脂糖改性物具有医学应用价值。目的:以活化降解琼脂糖/明胶可喷涂溶液为敷料,进行皮肤再生实验,探讨琼脂糖改性物作为皮肤再生修复敷料的可能性。方法:将活化琼脂糖和明胶按照一定配比制成总浓度3%的混合水溶液,过滤膜除菌,配制成活化琼脂糖(琼脂糖降解8h)和明胶将解的,并配制成1∶0,1∶1,1∶2,1∶3不同比例。取家兔4只,每只兔子背部选取对称点4个,共取16个实验点,分别将1∶1,1∶2,1∶3比例活化琼脂糖/明胶可喷涂凝胶喷涂于创面个4处;并以喷涂活化琼脂糖2处,单纯明胶2处做对照。术后4周观察喷涂敷料修复创面效果。结果与结论:术后7d,两种降解时间的活化琼脂糖和明胶配比1∶3的覆盖膜已经破裂,另外3个比例仍完好,4种比例创口均没有明显的感染发炎迹象,对比后发现敷料配比为1∶2的伤口愈合的最快。明胶的加入对伤口愈合的促进作用很明显。苏木精-伊红染色显示,皮肤创伤经活化降解琼脂糖/明胶复合物修复后的组织与正常组织类似。证实这类琼脂糖改性物具有辅助皮肤再生的功能。BACKGROUND：It has verified that seaweed polysaccharide-agarose modifiers have their medical application value.OBJECTIVE：To perform skin regeneration trials using sprayable activated agarose/gelatin solution,and to explore the possibility of agarose modifier as skin dressing for skin regeneration.METHODS：To prepare 3% sprayable mixture with the dissolved activated agarose and gelatin at a certain ratio,and then filtrated with millopore for sterilization to prepare activated agarose（agarose degradation for 8 hours）and gelatin degradation,and made into different ratios（1：0,1：1,1：2,1：3）.A total of four rabbits were obtained,and four sites were selected on the back of each rabbit,totally 16 experimental sites.The sprayable activated agarose/gelatin mixture（1：1,1：2,1：3）was directly sprayed on the four lesion sites.Sprayable activated agarose for two sites and simple gelatin for two sites served as controls.The effects of the wounds sprayed with dressing were observed at 4 weeks following surgery.RESULTS AND CONCLUSION：At 7 days following surgery,the cover film had broken in mixture of activated agarose and gelatin at 1：3,and remaining three were intact.No infection or inflammation occurred in wound of four ratios.Following comparison,the wound was rapidly healed in 1：2 ratio dressing.The additional gelatin showed promoting effects on wound healing significantly.Hematoxylin-eosin staining demonstrated that skin with the dressing was similar to autologous skin,which verified that sprayable activated agarose/gelatin have a promise in skin regeneration.

关 键 词：琼脂糖/明胶 明胶 可喷涂敷料 生物材料 海藻多糖 皮肤再生 

分 类 号：R318[医药卫生—生物医学工程]