幽门螺杆菌感染诱导C57BL/6小鼠动脉硬化的病理损伤及其在粥样斑块组织中的DNA分析  

Heficobacter pylori infection enhances atherosclerosis in high-cholesterol diet fed C57BL/6 mice

作　　者：陈星海[1] 王江滨[2] 王育珊[1] 刘忠民[1] 李岩[2] 

机构地区：[1]吉林大学第二医院急救医学科,长春130041 [2]吉林大学中日联谊医院消化内科

出　　处：《中华心血管病杂志》2010年第3期259-263,共5页Chinese Journal of Cardiology

基　　金：基金项目：国家自然科学基金（30471619）;吉林省科学技术委员会基金（20050408-2）

摘　　要：目的建立幽门螺杆菌（HP）感染的C57BL／6小鼠动脉粥样硬化模型，探讨HP感染在动脉粥样硬化发病机制中的作用。方法将48只C57BIf6小鼠随机分为4组，即HP灌饲联合高脂饮食组、单纯HP灌饲组、单纯高脂饮食组以及生理盐水对照组，每组12只小鼠。52周后观察4组小鼠主动脉粥样硬化发生率及其病理损伤程度、胃黏膜病理特征、血脂水平，并以PCR方法检测小鼠主动脉中HP尿素酶A（ureaseA，ureA）、尿素酶C（ureaseC，ureC）、细胞毒素相关基因A（cytotoxin．associatedgeneA，cagA）、空泡毒素A（vacuolatingcytotoxinA，vacA）的DNA表达。结果（1）单纯HP灌饲组和生理盐水对照组无动脉粥样硬化发生，而HP灌饲联合高脂饮食组和单纯高脂饮食组动脉粥样硬化发生率高达100％和91．6％，两组间差异无统计学意义。（2）HP灌饲联合高脂饮食组、单纯HP灌饲组、单纯高脂饮食组的总胆固醇（TC）、低密度脂蛋白（LDL）和甘油三酯（TG）水平高于生理盐水对照组（P均〈0．05），而高密度脂蛋白（HDL）水平低于生理盐水对照组（P均〈0．05）。且HP灌饲联合高脂饮食组的TC、LDL和TG水平高于单纯高脂饮食组，HDL水平低于单纯高脂饮食组，组问差异有统计学意义，P均〈0．05。（3）HP灌饲联合高脂饮食组Robe．s＆Thompson评分总分和斑块中泡沫细胞最多数目均高于单纯高脂饮食组，P均〈0．05。（4）12只HP灌饲联合高脂饮食组的小鼠动脉组织中有5例ureC检测出阳性，但未检测出urea、cagA和vacADNA。同时在其他3组小鼠动脉组织中均未检出HP的DNA表达。结论给C57BL／6小鼠灌饲HP可加重高脂饮食诱发的血脂代谢紊乱和动脉粥样硬化病理损伤程度，同时在HP感染的小鼠动脉粥样硬化组织中可检出HP的ureCDNA，提示HP感染与动脉粥样硬化的发生发展可能相关。Objective To evaluate the impacts of Helicobacter pylori （H. pylori ） infection on atherosclerosis and plasma lipid levels in high-cholesterol diet fed C57BL/6 mice. Method Female C57BL,/6 mice were randomly divided into4 groups （n = 12 each） ： fed with normal chow diet （A）, infected with H. pylori （ B）, fed with high-cholesterol diet （C） and infected with H. pylori and fed with high-cholesterol diet （D）. After 52 weeks, plasma levels of lipids were measured and aortic atherosclerosis was observed. The ureA, ureC, cagA and vacA DNA were also detected by PCR in the aortic arteries. Result （ 1 ） Prevalence of atherosclerosis was similar between group C and D （91.6% vs. 100%, P 〉0. 05） while there was no atherosclerosis in group A and B. H. pylori infected mice showed more obvious inflammation in gastric mueosa than mice without H. pylori infection. （2） The plasma levels of triglyceride, total cholesterol and LDL were higher and HDL was lower in group B, C and D than those in group A and in group D than in group C （all P 〈 0.05 ）. （ 3 ） Roberts ＆ Thompson scores and number of foam cells in plaques were significantly higher in group D compared with those in group C （ all P 〈 0. 05）. （4） ureC DNA was detected in 5 out of 12 aortic arteries of mice in group D but not in group A, B and C. Conclusion Our results suggested that H. pylori infection might enhance the atherosclerotic lesion formation in this mouse model.

关 键 词：动脉粥样硬化 螺杆菌 幽门 血脂异常 

分 类 号：R543[医药卫生—心血管疾病]