白念珠菌感染对小鼠皮肤Caspase-14表达及屏障功能的影响  

Effects of Candida Albicans Infection on Caspase-14 Expression in Mouse Skin

作　　者：李军[1] 陈谨萍 吴伽 

机构地区：[1]广州市妇女儿童医疗中心皮肤科,广东广州510120 [2]广州市妇女儿童医疗中心新生儿筛查中心,广东广州510120

出　　处：《皮肤性病诊疗学杂志》2010年第1期24-27,共4页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：广州市医学卫生科技项目(编号:416020101-2090018)

摘　　要：目的:检测小鼠皮肤白念珠菌感染时半胱氨酸蛋白酶-14(Caspase-14)的表达,探讨其对皮肤屏障的影响。方法:检测小鼠对照组正常皮肤(C)、受损皮肤(A1)、外用乳酸的受损皮肤(A2)、外用Caspase抑制剂Z-VAD-FMK的受损皮肤(A3)和接种白念珠菌的受损皮肤(A4)在实验前后pH值及经表皮水分流失量(TEWL)的变化。同时分别采用real-time PCR及western blotting检测各组小鼠皮肤的Caspase-14的表达,并比较其差异。结果:C、A1、A2、A3、A4各组处理后即刻及处理后12h的pH值为5.46±0.10、6.82±0.09、5.38±0.12、6.79±0.17、6.65±0.13和5.50±0.11、6.18±0.14、5.76±0.19、6.42±0.26、5.73±0.21,处理后12hTEWL值为27.2±7.3、39.2±8.2、51.0±9.1、47.4±8.5、58.5±9.6。C、A1、A2、A3、A4各组Caspase-14的转录水平分别为1.00±0.07、6.51±0.48、2.92±0.18、7.63±0.27、3.66±0.31,蛋白表达水平分别为1.00±0.12、5.17±0.38、3.24±0.27、6.31±0.33、3.02±0.24,各组间比较差异有统计学意义(P<0.05)。结论:受损皮肤Caspase-14的表达较正常皮肤明显增高,Caspase-14对受损皮肤屏障功能恢复具有重要作用;而白念珠菌感染可能通过降低皮肤表面pH值,下调受损皮肤Caspase-14的表达,从而影响皮肤屏障功能的恢复。Objective：To determine Caspase-14 expression in mouse skin with Candida albicans infection and explore the influence of the infection on mouse skin barrier. Methods：BALB/c mice were divided into 5 groups：normal skin group（C）、damaged skin group（A1）、 Lactobionic acid （LBA） on damaged skin group（A2）、Z-VAD-FMK on damaged skin group（A3） and Candida albicans on damaged skin group（A4）. Skin PH value and transepidermal water loss （TEWL） were measured. Caspase-14 expression in mouse skin was examined by real-time RT-PCR and western blotting. Results：The skin PH value of C、A1、A2、A3 、A4 immediately and 12 h after experiment were 5.46±0.10、6.82±0.09、5.38±0.12、6.79±0.17、6.65±0.13 and 5.50±0.11、6.18±0.14、5.76±0.19、6.42±0.26、5.73±0.21; TEWL of 12 hours after experiment were 27.2±7.3、39.2±8.2、51.0±9.1、47.4±8.5、58.5±9.6.Caspase-14 transcriptional levels of C、A1、A2、A3 、A4 were 1.00±0.07、6.51±0.48、2.92±0.18、7.63±0.27、3.66±0.31; Caspase-14 protein expression levels were 1.00±0.12、5.17±0.38、3.24±0.27、6.31±0.33、3.02±0.24. The statistical differences were significant（P0.05）.Conclusion：Caspase-14 expressed more in damaged skin than in normal skin; Caspase-14 is very important for skin rehabilitating. Candida albicans infection downregulated Caspase-14 expression in damaged skin probably by decreasing skin PH value and eventually affects skin rehabilitating.

关 键 词：白念珠菌 半胱氨酸蛋白酶-14 皮肤屏障 

分 类 号：R379[医药卫生—病原生物学]