去细胞全喉软骨支架的免疫学研究  

Immunological study on decellularized whole laryngeal scaffold

作　　者：马瑞娜[1] 崔鹏程[1] 侯楠[1] 罗家胜[1] 王兴[1] 

机构地区：[1]第四军医大学唐都医院耳鼻咽喉头颈外科,西安710038

出　　处：《中华耳鼻咽喉头颈外科杂志》2010年第3期233-237,共5页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：陕西省自然科学基金（2006C224）

摘　　要：目的评价去细胞全喉软骨支架的免疫原性。方法通过对兔离体喉行双侧颈总动脉顺行灌注去离子剂，构建去软组织细胞全喉支架12只。12只去细胞全喉软骨支架及12只新鲜喉分别埋植于24只青紫蓝兔喉旁肌内，于埋植后2、4、12、24周分别取材进行大体观察、组织学观察及淋巴细胞浸润计数比较。结果去细胞全喉软骨支架颜色透明苍白，完整地保留了喉的去细胞组织结构及软骨活性。去细胞全喉软骨支架埋植于青紫蓝兔喉旁肌后，各观察时间点未出现明显的免疫排斥反应，4周后埋植喉体积逐渐缩小，但软骨结构仍存在。对照组新鲜喉埋植2周时即出现明显的免疫排斥反应，4周后喉的形状完全消失，喉大部分结构已被溶解，被纤维结缔组织取代，周围组织出现不同程度的溶解坏死。同一时间点，对照组淋巴细胞浸润程度明显高于实验组，差异有统计学意义（2周与4周的t值分别为15．11和13．91，P值均〈0．01）。结论灌注法去细胞技术能够构建出具有低免疫原性的喉软骨支架，该支架有可能成为良好的喉修复材料。Objective To evaluate the immunogenicity of the decellularized laryngeal scaffold. Methods Twelve perfused, decelluarized laryngeal scaffolds were obtained from rabbits through common carotid artery perfusion with detergents. The twelve decellularized laryngeal scaffolds and the twelve fresh larynxes were then implanted in para-laryngeal muscles of rabbits and harvested after two weeks, four weeks, twelve weeks and twenty-four weeks, respectively. Macroscopic view, histological examination and lymphocyte infiltration test were performed. Results The decellularizcd larynxes were implanted and preserved the laryngeal extracellular matrix and laryngeal architecture. The decelluarized larynx did not show obvious immunological rejection after implanted into the para-laryngeal muscles of the reeipient rabbits. The volume of implanted larynx became smaller but retained cartilage scaffold. The larynxes in the control group presented the serious immunological rejection and the majority tissues of the larynxes were disintegrated and substituted by the fibrous connective tissues after four weeks. The peripheral tissues were damaged and necrotic at different degrees. The quantity of the lymphocyte infiltration in the control group was higher than that in the experiment group and the result had the statistical significance （ P 〈 0. 01 ）. Conclusions Perfused, decellularized technique can construct a low immune laryngeal cartilage scaffold which could be a satisfactory material for laryngeal repair.

关 键 词：喉 支架 灌注 局部 移植物排斥 

分 类 号：R318.08[医药卫生—生物医学工程]