Primary identifications and palynological observations of Perilla in China  

作　　者：Yan HU Li-Wei SUN Mokgolodi Neo C. Yan-Xia ZHANG Chun-Xiu WEN Xiao-Liang XIE Yu-Jun LIU 

机构地区：[1]College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China [2]Department of Biochemistry, Wenshan University, Wenshan 663000, China [3]Medicinal Herbs Research Center, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China

出　　处：《Journal of Systematics and Evolution》2010年第2期133-145,共13页植物分类学报（英文版）

基　　金：supported by the Scientific and Technological Project of Hebei Province of China:Studies on the Collection,Classification and Preservation of the endangered and Unadulterated Drug Germplasm Resources (grant no. 07276410D);as well as the project in 'Creation of Major New Drugs' under the National Major Projects for Science and Technology:Standards For Seeds,Seedlings and Planting (Breeding) of Medicinal Plants (grant no.2009ZX09308-002)

摘　　要：Unresolved controversies concerning the classification of the monotypic genus Perilla L. have hindered the complete understanding and subsequent sustainable use of these vital food, oil, and medicinal plants to their full potential. In the present study, we used scanning electron microscopy to obtain palynological evidence from 21 samples of Perilla plants from seven provinces in China as a potential further attribute for classification. The findings showed that pollen grains from plants of 11 samples were oblate, whereas those of the other l0 were suboblate; there were no prolate pollen grains observed. Pollen grains from all samples had diverse exine ornamentations. Based on whether they had continuous tecta on the ornamentations, the pollen grains from the 21 samples were classified into two categories： （i） 14 with irregular reticulates; and （ii） seven with continuous tecta with no perforations. None of the samples was bireticulate. The ornamentation pattern and size of pollen grains jointly provided evidence that it is appropriate for use in classifying the genus Perilla into five varieties of one species. Furthermore, by comparison, it is concluded that the shapes and exine ornamentations of Perilla are unique among those of the seven genera already investigated in the subfamily Lamioideae. Using these pollen features, Perilla can be easily distinguished from the two other subtribes （Menthinae Briq. and Thyminae Briq.） in the same tribe, supporting the view that Perilla and the other four related genera can be classified into one subtribe （Perillinae）.Unresolved controversies concerning the classification of the monotypic genus Perilla L. have hindered the complete understanding and subsequent sustainable use of these vital food, oil, and medicinal plants to their full potential. In the present study, we used scanning electron microscopy to obtain palynological evidence from 21 samples of Perilla plants from seven provinces in China as a potential further attribute for classification. The findings showed that pollen grains from plants of 11 samples were oblate, whereas those of the other l0 were suboblate; there were no prolate pollen grains observed. Pollen grains from all samples had diverse exine ornamentations. Based on whether they had continuous tecta on the ornamentations, the pollen grains from the 21 samples were classified into two categories： （i） 14 with irregular reticulates; and （ii） seven with continuous tecta with no perforations. None of the samples was bireticulate. The ornamentation pattern and size of pollen grains jointly provided evidence that it is appropriate for use in classifying the genus Perilla into five varieties of one species. Furthermore, by comparison, it is concluded that the shapes and exine ornamentations of Perilla are unique among those of the seven genera already investigated in the subfamily Lamioideae. Using these pollen features, Perilla can be easily distinguished from the two other subtribes （Menthinae Briq. and Thyminae Briq.） in the same tribe, supporting the view that Perilla and the other four related genera can be classified into one subtribe （Perillinae）.

关 键 词：classification cluster analysis PERILLA pollen grain scanning electron microscopy TAXONOMY variety. 

分 类 号：Q913.84[天文地球—古生物学与地层学]