软坚消瘿汤对甲状腺细胞凋亡及Bcl-2/Bax表达的影响  

Effect of Ruanjian Xiaoying Decoction on Apoptosis and Expressions of Bcl-2/Bax in Thyroid Cells

作　　者：方振伟[1] 张兰[1] 

机构地区：[1]辽宁中医药大学附属医院,沈阳100032

出　　处：《光明中医》2010年第3期422-424,共3页GUANGMING JOURNAL OF CHINESE MEDICINE

基　　金：辽宁省教育厅项目(No.20060543)

摘　　要：目的观察软坚消瘿汤对实验性自身免疫性甲状腺炎(EAT)大鼠甲状腺凋亡蛋白Bcl-2/Bax和细胞凋亡的影响,从细胞凋亡角度探讨其作用机制。方法运用甲状腺球蛋白与弗氏佐剂混合免疫注射法,联合饮用高碘水模拟大鼠实验性自身免疫性甲状腺炎。免疫组化染色法检测大鼠甲状腺细胞Bcl-2/Bax变化,原位末端标记技术检测细胞凋亡。结果软坚消瘿汤能显著降低Bax表达,增加甲状腺细胞Bcl-2表达,减少甲状腺细胞凋亡。结论软坚消瘿汤可能通过降低滤泡上皮细胞Bax表达,提高Bcl-2表达从而减轻甲状腺细胞凋亡。Objective：To evaluate the therapeutic effect of Xiaoying Ruanjian Decoction on experimental autoimmune thyroiditis （EAT） from the levels of apoptosis and apoptosis-regulated proteins Bcl-2/Bax of EAT rats. To Investigate the action Mechanism from apoptosis. Methods： An animal model of experimental autoimmune thyroiditis （EAT） rats ~bas developed by injecting thyroglobulin mixed with Freund＇s adjuvant immunization and drinking Periodate water. To observe the level change of Bcl-2/Bax in the thyroid of rat with Immunohistoehemieal staining. To detect the levels of apoptosis with TUNEL. Results： Ruanjian Xiaoying Decoction significantly reduces the levels of the expressions of Bax and apoptosis in thyroid ceils and increases the expression of Bcl-2. Conclusion： Ruanjian Xiaoying Decoction alleviates the levels of Bax in thyroid cells and increas the expression of Bcl-2 by the suppression of relative expressions of antibodies in serum levels of EAT, which reduces apoptosis in thyroid cells.

关 键 词：软坚消瘿汤 实验室自身免疫性甲状腺炎 BCL-2/BAX 细胞凋亡 中医实验医学 中药药理学 

分 类 号：R285.5[医药卫生—中药学]