乙肝患者e抗原定量与HBV-DNA及ALT的相关性分析  

The correlative analysis among HBeAg,HBV-DNA and ALT in persons infected by hepatitis B virus

作　　者：钟乔华[1] 陈尤佳[1] 谢细娜[1] 姚宝胜[1] 

机构地区：[1]广东省揭阳市人民医院检验科,522000

出　　处：《中国实用医药》2010年第9期39-40,共2页China Practical Medicine

摘　　要：目的分析e抗原定量与HBV-DNA及ALT的相关性,探讨三者之间的关系。方法应用时间分辨荧光免疫分析法和荧光定量PCR法及丙氨酸氨基转移酶法分别对226例乙肝患者血清e抗原和HBVDNA及ALT进行测定,并按e抗原测定结果分成5组。结果①乙肝患者血清e抗原定量与HBV-DNA含量呈等级相关,(相关系数0.514,P<0.001),并且e抗原在0.03～3.75 NCU/ml之间HBV-DNA含量随e抗原定量的升高而升高,而e抗原定量在3.75 NCU/ml以上时与HBV-DNA含量关系不大(Ⅳ组与V组比较P>0.05);②e抗原定量与ALT水平之间不存在相关关系。结论e抗原定量与HBV-DNA含量有很好的一致性,可反映体内HBV的复制情况,但e抗原定量不能代替肝功能的检测,只有同时检测e抗原定量和ALT水平才能为判断HBV感染、复制及肝功能损伤程度提供有价值的诊断依据。Objective To explore the relationship between HBeAg concentration with HBV-DNA and ALT value.Methods The quantity of HBV-DNA and serum HBeAg,ALT concentration of 226 hepatitis B patients were detected by fluorescence quantitative PCR（FQ-PCR）and time-resolved fluorescence immunity analysis systematic（TRIFA）and enzyme respectively at the same time.5 groups were divided according to their HBeAg results.Results ①The levels of HBeAg and HBV-DNA showed rank correlation（0.514,P0.001）,and the HBV-DNA was increased with increasing concentration of HBeAg levels between 0.03~3.75 NCU/ml.But when the HBeAg concentration above 3.75 NCU/ml,the relationship between HBeAg and HBV-DNA showed no statistically different.②HBeAg had no correlation with ALT.（P0.05）.Conclusion HBeAg is identical tendency with HBV-DNA,and it can be used to reflect the HBV replication,but can not take the place of the hepatic function detected.So analyzing HBeAg and ALT together would be advisable to the infection of HBV、the virus replication statement and the hepatic function degree of injury.

关 键 词：乙型肝炎病毒 e抗原定量 HBV-DNA 丙氨酸氨基转移酶 

分 类 号：R512.62[医药卫生—内科学]