耐甲氧西林金黄色葡萄球菌杀白细胞毒素基因的检测  

Research on the Panton-Valentine Leukocidin Gene of Methicillin-resistant Staphylococcus Aureus

作　　者：张征[1] 刘荣欣[1] 孙静娜[1] 赵小洁[1] 王政民[1] 赵建宏[2] 

机构地区：[1]河北医科大学第一医院,石家庄050031 [2]河北医科大学第二医院,石家庄050000

出　　处：《中国中医药现代远程教育》2010年第3期177-178,共2页Chinese Medicine Modern Distance Education of China

基　　金：河北省科学技术研究与发展指导计划[No:052761136]

摘　　要：目的了解耐甲氧西林金黄色葡萄球菌杀白细胞毒素基因的检出情况。方法收集2007年1月至2008年6月分离自河北医科大学第一医院临床患者的MRSA45株,采用聚合酶链式反应进行杀白细胞毒素基因的检测。结果45株MRSA中有12株检出杀白细胞毒素基因,检出率为26.7%。结论杀白细胞毒素基因PVL检出率较高,是MRSA的重要致病因子。Objective To learn the Panton-Valentine Leukocidin gene of Methicillin resistant Staphylococcus aureus （MRSA）. Methods 45 strains of MRSA were clinically isolated from the patients in The First Hospital of HeBei Medical University from January 2007 to June 2008.The Panton-Valentine Leukocidin gene PVL were detected by polymerase chain reaction（PCR） methods. Resu Its Among 45 strains of MRSA, the rate of positive of the Panton-Valentine Leukocidin gene were 26.7%（12/45）. Conclusion The Panton-Valentine Leukocidin gen e in Multiple drug-resistance MRSA were higher than. PVL was important causative agent of MRSA.

关 键 词：耐甲氧西林金黄色葡萄球菌 MRSA 杀白细胞毒素基因 PVL基因 

分 类 号：R378[医药卫生—病原生物学]