结核灶内抗酸菌L型检出方法的研究和意义  

Study and significance of method on detecting acid-fast bacterial L-form in tuberculosis fous

作　　者：郭安河[1] 程慧[1] 张国昌[2] 

机构地区：[1]蚌埠铁路中心医院病理科 [2]石河子大学医学院病理教研室

出　　处：《农垦医学》1998年第3期131-133,共3页Journal of Nongken Medicine

摘　　要：本文应用Itenfisedkinyoun（IK）改良抗酸染色，Ziehl－Neelsen（ZN）经典抗酸染色和BacillusCalmetteGuevin（BCG）抗体免疫组化染色等方法对181例结核病组织切片进行了结核杆菌的检测．结果IK抗酸染色法L型结核菌检出率为67．4％，ZN法为29．8％，两者差异有显著性。两种抗酸染色法均证明结核灶内主要（67．4％、29．8％）为抗酸菌L型；杆菌型仅1例，对两种抗酸染色均呈阳性反应。BCG众体免疫组化染色发现82．3％结核灶内有其抗原存在。提示抗酸菌L型可能是结核病误诊、复发和播散的重要原因。IK法可提高抗酸菌L型的检出率。181 cases of tuberculosis were studied using Intenfised kinyoun (IK), Ziehl - Neelsen (ZN) acid - fast staining and Bacillus Calmette Guerin (BCG ) antibody immunohistochemical technique(ABC). It was showed that the detective rate of acid - fast bacterial L - form was 67. 4% (IK method ), 29. 8 % (ZN method ), which are obviously different, P<0. 005. Two acid - fast staining results indicated that acid - fast bacterial L - forms was mainly in tuberculosls focus (67. 4 %, 28. 9 % );the positive of acid-fast bacillus was only one case (both IK and ZN ). The positive rate of L - form antigen was 82. 3 %. The results indicated that the infection of acid -fast bacterial L-forms might be important causes of misdiagnosis, recurrence and spread. The IK method possibly increases detecting rate of acid - fast bacterial L - forms.

关 键 词：IK 抗酸染色 ZN BCG 结核病 抗酸菌L型 

分 类 号：R520.4[医药卫生—内科学] R446[医药卫生—临床医学]