高效液相色谱法测定可乐宝林片中盐酸可乐定的含量  

Determination of Clonidine Hydrochloride in Compound Clonidine Hydrochloride Tablets by RP-HPLC

作　　者：马永贵[1] 李自明[1] 闵敏[1] 贺国芳[1] 汪秋兰[1] 王文清[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,武汉430030

出　　处：《医药导报》2010年第4期522-524,共3页Herald of Medicine

摘　　要：目的建立可乐宝林片中盐酸可乐定的含量测定方法。方法采用高效液相色谱法,色谱柱:HypersilC18(4.6mm×250mm,5μm);流动相:0.22%辛烷磺酸钠-甲醇-磷酸(400:600:1,用1mol·L-1氢氧化钠溶液或磷酸调pH值至3.0),流速0.9mL·min-1;检测波长:220nm。结果盐酸可乐定在1.37～6.85μg.mL-1的浓度范围内线性关系良好(r=1.0000);盐酸可乐定的平均回收率分别为100.5%(RSD=0.76%),100.6%(RSD=2.66%),101.9%(RSD=0.92%)(n=3)。结论该法灵敏快速、准确可靠,可作为可乐宝林片中盐酸可乐定的含量测定方法。Objective To establish a RP-HPLC method for determination of clonidine hydrochloride in compound clonidine hydrochloride tablets.Methods Chromatography was carried out on a column of the Hypersil C18（4.6 mm×250 mm,5 μm）;mobile phase：0.22% sodium 1-octanesulfonate-methanol-phosphoric acid（400：600：1,pH=3.0）;flow rate：0.9 mL·min-1 and the detection wavelength was 220 nm.Results A good linearity of clonidine hydrochloride was obtained within 1.37-6.85 μg·mL-1;the average recoveries of clonidine hydrochloride was 100.5%（RSD=0.76%）,100.6%（RSD=2.66%）,101.9%（RSD=0.92%）（n=9）,respectively.Conclusion The method is simple,rapid,sensitive and accurate.It can be used to control quality of clonidine hydrochloride in compound clonidine hydrochloride tablets.

关 键 词：可乐定 盐酸 可乐宝林片 含量测定 

分 类 号：R927.2[医药卫生—药学] R985